AI Article Synopsis
- Aspernidine A is a compound derived from the fungus Aspergillus nidulans, specifically a type of alkaloid called prenylated isoindolinone.
- Researchers created a genome-wide kinase knockout library of A. nidulans and discovered that deleting the mpkA gene leads to the production of aspernidine A.
- Further gene deletions in the kinase knockout background helped identify the specific gene cluster responsible for the biosynthesis of aspernidine A, and intermediates from mutant strains were isolated to better understand the biosynthesis pathway.
Article Abstract
Aspernidine A is a prenylated isoindolinone alkaloid isolated from the model fungus Aspergillus nidulans. A genome-wide kinase knockout library of A. nidulans was examined, and it was found that a mitogen-activated protein kinase gene, mpkA, deletion strain produces aspernidine A. Targeted gene deletions were performed in the kinase deletion background to identify the gene cluster for aspernidine A biosynthesis. Intermediates were isolated from mutant strains which provided information about the aspernidine A biosynthesis pathway.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713076 | PMC |
| http://dx.doi.org/10.1021/ol401187b | DOI Listing |
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