Considering the development of the need for small volume and light weight of spectral imaging systems on airborne platform, the characters of several main imaging spectrometers was analyzed and the present paper focuses on introducing imaging spectrometers featuring a grating or a prism disperser. A compact hyperspectral imaging system based on Offner relay configuration is reported. Combining the characters of the concentric Offner relay, and knowing the system design parameters, two novel kinds of spherical spectrometer systems using dispersive elements in divergent and convergent beam were designed. Moreover, the system MTF, spot diagram and the curves of smile and keystone were analyzed. The results show that these two kinds of Offner spectrometer achieve the purpose of a compact form of remote sensing system with excellent imaging quality close to diffraction limit. At the same time, decreasing the smile and keystone to a small (0.1) fraction of a pixel assures uniformity of acquiring spectral data.
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Eur J Neurosci
July 2024
Institute of Animal Physiology, Department of Animal Physiology and Molecular Biomedicine, Justus Liebig University Gießen, Gießen, Germany.
Across vertebrate species, the olfactory epithelium (OE) exhibits the uncommon feature of lifelong neuronal turnover. Epithelial stem cells give rise to new neurons that can adequately replace dying olfactory receptor neurons (ORNs) during developmental and adult phases and after lesions. To relay olfactory information from the environment to the brain, the axons of the renewed ORNs must reconnect with the olfactory bulb (OB).
View Article and Find Full Text PDFBiomed Opt Express
September 2018
Department of Experimental Psychology, University of Oxford, 15 Parks Road, Oxford OX1 3PH, UK.
The adaptive optics scanning laser ophthalmoscope (AOSLO) was first developed in 2002 and since then the technology has been adopted in several laboratories around the world, for both clinical and psychophysical research. There have been a few major design implementations of the AOSLO. The first used on-axis tilted spherical mirrors in a planar arrangement, and the second minimized the build up of astigmatism present in the first design by using a non-planar arrangement.
View Article and Find Full Text PDFMethods Mol Biol
May 2019
Department of Animal Physiology and Molecular Biomedicine, Institute of Animal Physiology, Justus-Liebig-University Giessen, Giessen, Germany.
Electroporation is an efficient method of transferring charged macromolecules into living cells in order to study their morphology, function, and connectivity within neuronal networks. Labeling cells with fluorophore-coupled macromolecules can be used to trace projections of whole neuronal ensembles, as well as the fine morphology of single cells. Here, we present a protocol to visualize pre- and postsynaptic components of a sensory relay synapse in the brain, using the olfactory system of Xenopus laevis tadpoles as a model.
View Article and Find Full Text PDFIn this work, we present a close-range ultraviolet imaging spectrometer with high spatial resolution, and reasonably high spectral resolution. As the transmissive optical components cause chromatic aberration in the ultraviolet (UV) spectral range, an all-reflective imaging scheme is introduced to promote the image quality. The proposed instrument consists of an oscillating mirror, a Cassegrain objective, a Michelson structure, an Offner relay, and a UV enhanced CCD.
View Article and Find Full Text PDFConventional lens-based image relays introduce radial group delay that significantly reduces focal-spot intensity of ultra-broadband short pulses. A direct compensation scheme that can be used with conventional singlet image relays is proposed. The compensation scheme is based on using two negative lenses embedded within an Offner imaging system.
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