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Elucidation of peptide screen for targeted identification of Yersinia pestis by nano-liquid chromatography tandem mass spectrometry.

Sci Rep

January 2025

Microbiology Division, Defence Research and Developmental Establishment, Jhansi Road, Gwalior, 474002, India.

Yersinia pestis, a Gram-negative bacterium is the causative agent of the fatal communicable disease plague. The disease had a profound impact on human history. Plague bacteria are usually transmitted to humans through the bite of an infected rat flea.

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Background: Fleas are insect vectors that transmit several Gram-negative bacterial pathogens acquired by ingesting infected vertebrate blood. To combat foodborne illness, insect midgut epithelial cells are armed with efficient microbial recognition and control systems, such as the immune deficiency (IMD) pathway that regulates the expression of antimicrobial peptides (AMPs). However, despite their medical and veterinary importance, relatively little is known about the IMD signaling pathway and production of AMPs in the digestive tract of cat fleas (Ctenocephalides felis).

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Metabolism and immune responses of striped hamsters to ectoparasite challenges: insights from transcriptomic analysis.

Front Immunol

December 2024

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Introduction: The striped hamster, often parasitized by ectoparasites in nature, is an ideal model for studying host-ectoparasite molecular interactions. Investigating the response to ectoparasites under laboratory conditions helps elucidate the mechanism of host adaptations to ectoparasite pressure.

Methods: Using transcriptome sequencing, we analyzed gene expression in striped hamsters after short-term (3 days) and long-term (28 days) flea () parasitism.

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Phylogeny and divergence time estimation of the subfamily Amphipsyllinae based on the mitogenome.

Front Vet Sci

December 2024

Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors, Dali University, Dali, China.

Fleas are primarily parasites of small mammals and serve as essential vectors of the transmission of plague. The subfamily Amphipsyllinae (Siphonaptera: Leptopsyllidae) consists of 182 species across 13 genera, widely distributed worldwide. Only two species of Amphipsyllinae have been sequenced for complete mitogenomes to date.

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Palaearctic flea beetle (Curtis) (Coleoptera, Chrysomelidae, Galerucinae), herbivore of (garlic mustard), new to North America.

Biodivers Data J

December 2024

Systematic Entomology Laboratory, MRC-168 Washington, United States of America Systematic Entomology Laboratory MRC-168 Washington United States of America.

Background: The univoltine leaf beetle (Curtis, 1837b) is native to the Palaearctic Region from Japan to western Europe.This species was previously evaluated as a potential biological control agent against invasive populations of the woodland weed (Bieb.) Cavara & Grande (Brassicaceae) in North America, but rejected because it could harm native and at-risk populations of Brassicaceae.

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