AI Article Synopsis
- Staphylococcus aureus infections in hospitals now lead to more deaths annually in the US than HIV/AIDS, with a concerning rise in community-associated methicillin-resistant strains (CA-MRSA).
- The study aimed to develop a method to analyze autoinducing peptide I (AIP-I), crucial for the bacterial agr quorum-sensing system, using advanced UHPLC coupled with mass spectrometry techniques.
- The developed method successfully quantified AIP-I in S. aureus cultures, revealing that production peaks after about 16 hours and correlates with virulence in some strains, indicating a potential link between AIP-I levels and infection severity.
Article Abstract
Staphylococcus aureus infections acquired in hospitals now cause more deaths per annum in the US than does HIV/AIDS. Perhaps even more alarming is the rise in community associated methicillin-resistant S. aureus (CA-MRSA) infections, which have spread out of hospital settings and are infecting otherwise healthy individuals. The mechanism of enhanced pathogenesis in CA-MRSA remains unclear, but it has been postulated that high activity in the agr quorum-sensing system could be a contributing factor. The purpose of this study was to develop a quantitative method for analysis of autoinducing peptide I (AIP-I), the activating signal for the agr system in S. aureus. An effective method was developed using ultrahigh performance liquid chromatography (UHPLC) coupled to electrospray ionization mass spectrometry with an LTQ Orbitrap mass spectrometer. Relying on the exceptional resolving power and mass accuracy of this instrument configuration, it was possible to quantify AIP-I directly from the complex growth media of S. aureus cultures with a limit of detection (LOD) of 0.25μM and a linear dynamic range of 2.6 to 63μM. The method was then employed to monitor time-dependent production of AIP-I by S. aureus cultures, and it was observed that AIP-I production reached a maximum and leveled off after approximately 16h. Finally, it was determined that virulence of S. aureus was correlated with AIP-I production in some (but not all) strains analyzed.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717363 | PMC |
| http://dx.doi.org/10.1016/j.jchromb.2013.04.019 | DOI Listing |
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