AI Article Synopsis
- Pten, a tumor suppressor gene, is crucial in the female reproductive system, but its role in polycystic ovaries (PCOS) remains unclear.
- Researchers developed a rat model of PCOS and found significantly increased levels of pten mRNA and PTEN protein in polycystic ovary tissues.
- The study suggests that pten down-regulation using shRNA may help inhibit the development of polycystic ovaries, providing new insights into PTEN's role in female reproductive disorders.
Article Abstract
Pten (phosphatase and tensin homolog deleted on chromosome 10), a kind of tumor suppressor gene, plays important roles in female reproductive system. But its expression and roles in the formation of polycystic ovaries are yet to be known. In this study, we constructed a rat model of PCOS using norethindrone and HCG injections and found the expressions of pten mRNA and PTEN protein increased significantly in the polycystic ovary tissue by immunohistochemistry, RT-PCR, and western blot. Furthermore, the results showed that in vivo ovaries could be effectively transfected by lentiviral vectors through the ovarian microinjection method and indicated that pten shRNA may inhibit the formation of polycystic ovaries by pten down-regulation. Our study provides new information regarding the role of PTEN in female reproductive disorders, such as polycystic ovary syndrome.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695674 | PMC |
| http://dx.doi.org/10.1007/s11010-013-1673-z | DOI Listing |
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