An electrochemical magneto immunosensor for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The immunological reaction is performed on magnetic beads (MBs) as a solid support in which the transglutaminase enzyme (TG2) is covalently immobilized (TG2-MB) and then ATG2 were revealed by an antibody labeled with peroxidase. The electrochemical response of the enzymatic reaction with o-phenilendiamine and H₂O₂ as substrates by square wave voltammetry was correlated with the ATG2. Graphite-epoxi composite cylindrical electrodes and screen printed electrodes were used as transducers in the immunosensor. A total number of 29 sera from clinically confirmed cases of celiac disease and 19 negative control sera were tested by the electrochemical magneto immunosensor. The data were submitted to the receiver-operating characteristic plot (ROC) analysis which indicated that 16.95 units was the most effective cut-off value (COV) to discriminate correctly between celiac and non-celiac patients. Using this point for prediction, sensitivity was found to be 100%, while specificity was 84%.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2013.04.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Integrated metabolomics and proteomics analysis of anthocyanin biosynthesis regulations in passion fruit (Passiflora edulis) pericarp.
Plant Physiol Biochem
December 2024
Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China.
Anthocyanin is the primary color-developing component in the pericarp of the passion fruit. Although the pericarp of the passion fruit is anticipated to be a significant source of anthocyanin, however, information regarding anthocyanin biosynthesis in the passion fruit pericarp remains unexplored. Based on metabolomics analysis, a total of five anthocyanins were identified in the purple-skinned passion fruit pericarp, among which three anthocyanins, petunidin-3-O-arabinoside, geranylgeranyl-3,5-O-diglucoside, and petunidin-3-O-rutinoside, play key roles in the coloration of the passion fruit pericarp.
View Article and Find Full Text PDFDisposable and sensitive electrochemical magneto-immunosensor for point-of-care HCV diagnostics: Targeting HCVcAg, the active viremia biomarker, in patient samples.
Biosens Bioelectron
December 2024
Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, United Kingdom. Electronic address:
Early detection of hepatitis C virus (HCV) infection is crucial for eliminating this silent killer, especially in resource-limited settings. HCV core antigen (HCVcAg) represents a promising alternative to the current "gold standard" HCV RNA assays as an active viremia biomarker. Herein, a highly sensitive electrochemical magneto-immunosensor for the HCVcAg was developed.
View Article and Find Full Text PDFElectrochemical and Optical Multi-Detection of Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor.
Biosensors (Basel)
December 2024
Department of Electrical-Electronics Engineering, Abdullah Gul University, Kayseri 38039, Türkiye.
detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In this work, we present a paper-based electrochemical biosensor utilizing magnetic core-shell FeO@CdSe/ZnS quantum dots (MQDs) to achieve fast detection, low cost, and high sensitivity.
View Article and Find Full Text PDFElectrochemically superior ammonia production from electroplating wastewater over oxygen-incorporated antiperovskite.
J Colloid Interface Sci
December 2024
China Auto Information Technology Co., Ltd, Tianjin 300300, People's Republic of China.
Article Synopsis
- The electrochemical reduction of nitrates to ammonia from electroplating wastewater is a promising method for environmental sustainability, but the slow charge transfer hampers efficiency.
- A new oxygen-incorporated CuNFe antiperovskite was created using magneto-thermal stimulation, achieving a notable ammonia yield of 1.34mmol h cm and a Faradaic efficiency of 95.5% at -0.6 V.
- A novel membraneless flow electrolyzer was also developed, allowing for the simultaneous reduction of nitrates and recovery of ammonia, making it a practical solution for treating real electroplating wastewater.
Magnetic Effects in Electrocatalysis Studied with Electrochemical Impedance Spectroscopy: The Cases of Oxygen Reduction and Evolution Reactions on Cobalt Ferrite Electrodes.
ChemSusChem
December 2024
Departament de Química Física i Institut Universitari d'Electroquímica, Universitat d'Alacant, Apartat 99, E-03080, Alicante, Spain.
Recent years have witnessed an intense effort to unravel magnetic field effects in electrocatalysis, as they can enhance the performance of common electrocatalysts. Both experimental and theoretical studies have shown that magnetic fields may affect, among others, the macroscopic spin-orbital ordering, charge transport, bubble release, and electron transfer kinetics. This paper highlights Electrochemical Impedance Spectroscopy (EIS) as a tool to analyze and separate the effects of magnetic field on both the oxygen reduction and evolution reactions at cobalt iron oxide electrodes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!