Culturing oligodendrocyte lineage cells from neonatal rats.

Methods Mol Biol

Department of Neuroscience and Cell Biology, Graduate School of Biochemical Sciences, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.

Published: December 2013

The use of enriched oligodendrocyte lineage cell cultures has yielded insight into functions of these cells and regulatory mechanisms. This chapter details methods that result in such cultures.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-62703-444-9_8DOI Listing

Publication Analysis

Top Keywords

oligodendrocyte lineage
8
culturing oligodendrocyte
4
lineage cells
4
cells neonatal
4
neonatal rats
4
rats enriched
4
enriched oligodendrocyte
4
lineage cell
4
cell cultures
4
cultures yielded
4

Similar Publications

Deletion and duplication in the human 16p11.2 chromosomal region are closely linked to neurodevelopmental disorders, specifically autism spectrum disorder. Data from neuroimaging studies suggest white matter microstructure aberrations across these conditions.

View Article and Find Full Text PDF

Single-Cell Insights Into Cellular Response in Abdominal Aortic Occlusion-Induced Hippocampal Injury.

CNS Neurosci Ther

January 2025

Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder, Capital Medical University, Beijing, China.

Objective: Ischemia-reperfusion of the abdominal aorta often results in damage to distant organs, such as the heart and brain. This cellular heterogeneity within affected tissues complicates the roles of specific cell subsets in abdominal aorta occlusion model (AAO) injury. However, cell type-specific molecular pathology in the hippocampus after ischemia is poorly understood.

View Article and Find Full Text PDF

The pericellular function of Fibulin-7 in the adhesion of oligodendrocyte lineage cells to neuronal axons during CNS myelination.

Biochem Biophys Res Commun

January 2025

Department of Molecular and Cellular Biology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan; Department of Clinical Bioanalysis and Molecular Biology, Graduate School of Medical and Dental Sciences, Institute Science of Tokyo/TMDU, Tokyo, Japan. Electronic address:

Myelin is an electrical insulator that enables saltatory nerve conduction and is essential for proper functioning of the central nervous system (CNS). It is formed by oligodendrocytes (OLs) in the CNS, and during OL development various molecules, including extracellular matrix (ECM) proteins, regulate OL differentiation and myelination; however, the role of ECM proteins in these processes is not well understood. Our present work is centered on the analyses of the expression and function of fibulin-7 (Fbln7), an ECM protein of the fibulin family, in OL differentiation.

View Article and Find Full Text PDF

Molecular and cellular dynamics of the developing human neocortex.

Nature

January 2025

The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, USA.

The development of the human neocortex is highly dynamic, involving complex cellular trajectories controlled by gene regulation. Here we collected paired single-nucleus chromatin accessibility and transcriptome data from 38 human neocortical samples encompassing both the prefrontal cortex and the primary visual cortex. These samples span five main developmental stages, ranging from the first trimester to adolescence.

View Article and Find Full Text PDF

To myelinate axons, oligodendrocyte precursor cells (OPCs) must stop dividing and differentiate into premyelinating oligodendrocytes (preOLs). PreOLs are thought to survey and begin ensheathing nearby axons, and their maturation is often stalled at human demyelinating lesions. Lack of genetic tools to visualize and manipulate preOLs has left this critical differentiation stage woefully understudied.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!