Measurement of phospholipase C (PLC) activity in vitro is a valuable biochemistry technique easily applicable in samples from different organisms. It quantifies the enzymatic activity of a key protein involved in critical developmental functions in organisms such as plants, animals, and bacteria. A protocol is described which assays the formation of two main products of the PLC hydrolysis reaction on radioactively labeled phospholipid substrates.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-62703-401-2_17 | DOI Listing |
Publication Analysis
Top Keywords
activity vitro
8
determination phospholipase
4
phospholipase activity
4
vitro measurement
4
measurement phospholipase
4
phospholipase plc
4
plc activity
4
vitro valuable
4
valuable biochemistry
4
biochemistry technique
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!