AI Article Synopsis
- Hematopoietic stem cell transplantation (HSCT) often leads to viral reactivations, necessitating regular viral load monitoring for early diagnosis and preemptive therapy.
- A study focused on tracking perforin expression in CD8+ T-cells in children post-HSCT, finding that those with viral reactivations had significantly higher levels of perforin-expressing CD8+ T-cells.
- The increase in perforin expression was closely linked to a decrease in viral load, suggesting that measuring perforin levels could serve as a useful indicator of effective antiviral T-cell recovery in the early stages following HSCT.
Article Abstract
Hematopoietic stem cell transplantation (HSCT) is frequently complicated by viral reactivations. Early diagnosis of viral reactivations and preemptive therapy relies on frequent viralload monitoring. An easy marker of effective cytotoxicity in lymphopenia is lacking and therefore we studied perforin-expression in CD8+T-cells in children following HSCT. Prospectively, we weekly monitored viral loads and perforin-expression of CD8+T-cells in whole blood by FACS, until 4months after HSCT in children. 27 patients were included (median age 4,3, range 0.3-20,1years) of whom 19 developed viral reactivations. These patients showed higher percentages of perforin-expressing CD8+T-cells (17,2%, range 0-63%) than those without (6,8%; range 0-16%) (p=0.001). The increased percentage of perforin-expressing CD8+T-cells coincided with a decrease in viral load with a median interval between maximum viral load and maximum level of perforin-expression of 0,4weeks (range 0.1-7.1). We conclude that perforin-expression in CD8+T-cells may be a marker for effective antiviral T-cell reconstitution early after HSCT in children.
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| http://dx.doi.org/10.1016/j.clim.2013.02.023 | DOI Listing |
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