AI Article Synopsis
- The study addresses the challenge of studying Y chromosome genes due to insufficient mouse models with specific mutations.
- Researchers used TALEN gene editing technology in mouse embryonic stem cells to create mice with disruptions and insertions in the Sry and Uty genes on the Y chromosome.
- This approach demonstrates the effectiveness of TALEN in exploring the biological functions of Y-linked genes.
Article Abstract
The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681814 | PMC |
| http://dx.doi.org/10.1038/nbt.2595 | DOI Listing |
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