Metabolic flux network analysis of fermentative hydrogen production: using Clostridium tyrobutyricum as an example.

Bioresour Technol

Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

Published: August 2013

AI Article Synopsis

  • This study uses metabolic flux network analysis (MFA) to investigate hydrogen production from Clostridium tyrobutyricum using glucose or lactate/acetate.
  • The results indicate that hydraulic retention time (HRT) significantly influences hydrogen production; specifically, varying HRT affects the balance of key metabolites like lactate, butyrate, and acetate.
  • The findings suggest that MFA can be an effective tool for optimizing and designing processes for fermentative hydrogen production.

Article Abstract

This study applies metabolic flux network analysis (MFA) to evaluate the metabolic flux of fermentative hydrogen production (FHP) with the use of Clostridium tyrobutyricum fed with either glucose or lactate/acetate as substrates. The MFA results suggest that hydraulic retention time (HRT) presents significant impact on hydrogen production from glucose. At HRT between 4 and 18 h, increase of HRT increased hydrogen production but decreased lactate production, while at HRT below 4 h decrease of HRT increased hydrogen production but decreased lactate production. The flux for lactate, butyrate and acetate seemed to affect H₂ production, due presumably to their impacts on the balance of NADH, ferredoxin and ATP. It is suggested that the MFA can be a useful tool to provide valuable information for optimization and design of the fermentative hydrogen production process.

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http://dx.doi.org/10.1016/j.biortech.2013.03.141DOI Listing

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