AI Article Synopsis
- A new gasometer device for total CO2 measurement in biological fluids was tested and showed promising results, including a linear range of 10 to 40 mM/l and the ability to conduct 20 tests in just 35 minutes.
- It demonstrated high reliability with a low coefficient of variation (1.6%) over multiple tests and good daily reproducibility within a margin of 2 mM/l.
- Correlation with established methods, such as a colorimetric method and calculations from pH and pCO2, was strong (r = 0.90 and r = 0.92 respectively), but the technique poses challenges in pediatric cases due to a minimum plasma sample requirement of 0.5 ml.
Article Abstract
It seemed to us interesting to study the results supplied by a new apparatus designed for gasometric estimation of total CO2 in biological fluids according to a technique derived from that of Van Slyke. The main qualities observed are: linearity (10 to 40 mM/l); rapidly (20 estimations in 35 minutes), reliability (coefficient of variation 1.6% over 15 estimations), daily reproducibility (2 standard deviations = 2 mM/l), satisfactory correlation with a colorimetric method using orthocresolphtalein adapted to SMA 6/60 (r = 0.90) and with an indirect method leading to total CO2 levels by calculation from pH and pCO2 (r = 0.92). However, this technique is difficult to use in pediatrics for the samples of plasma cannot be less than 0.5 ml.
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