AI Article Synopsis
- Researchers used liquid chromatography-mass spectrometry (LC-MS) to measure levels of juvenile hormones (JH) I, II, and III in the hemolymph of silkworms, specifically transforming them into JH methoxyhydrines (JH-MHs) for better analysis.
- The addition of sodium acetate during the analytical process improved the detection sensitivity of each JH-MH, allowing for effective monitoring throughout key developmental stages of the silkworm.
- Results showed a peak of JH I right after molting in the 3rd and 4th instar stages, followed by a sharp decline on day 1 before ecdysis, with no significant increase noted until the pupal stage began.
Article Abstract
Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.
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| http://dx.doi.org/10.1271/bbb.120883 | DOI Listing |
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