AI Article Synopsis
- Advances in optical imaging technologies and genetically encoded fluorescent proteins allow for long-term visualization of stem cells in living organisms and lab settings.
- The development of fluorescent protein reporters linked to specific proteins helps to target and identify individual cells within mixed populations.
- This technology enables detailed observation of cell behaviors like division, movement, and death, facilitating the tracking of cell lineages over time.
Article Abstract
Advances in optical imaging technologies combined with the use of genetically encoded fluorescent proteins have enabled the visualization of stem cells over extensive periods of time in vivo and ex vivo. The generation of genetically encoded fluorescent protein reporters that are fused with subcellularly localized proteins, such as human histone H2B, has made it possible to direct fluorescent protein reporters to specific subcellular structures and identify single cells in complex populations. This facilitates the visualization of cellular behaviors such as division, movement, and apoptosis at a single-cell resolution and, in principle, allows the prospective and retrospective tracking towards determining the lineage of each cell.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967584 | PMC |
| http://dx.doi.org/10.1007/7651_2013_19 | DOI Listing |
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