Structural determination and NMR characterization of a bacterial exopolysaccharide.

Int J Biol Macromol

Biochemistry Laboratory, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia.

Published: August 2013

A strain of Bacillus licheniformis with high exopolysaccharide (EPS) production ability was isolated and identified. A new type of EPS was isolated from the strain fermentation and its structural characteristics were investigated and elucidated by partial and total acid hydrolysis, Fourier transform infrared, and (1)H and (13)C NMR spectroscopy including 2D (1)H, COSY, NOESY, XHCOR and HMBC experiments. Based on obtained data, the EPS was found to be a levan composed of linear chains of (2→6)-linked β-d-fructofuranosyl residues with connections β (2→6).

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http://dx.doi.org/10.1016/j.ijbiomac.2013.04.036DOI Listing

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