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Rapid and accurate demultiplexing of direct RNA nanopore sequencing datasets with SeqTagger.
Genome Res
January 2025
Center for Genomic Research (CRG), Universitat Pompeu Fabra (UPF), ICREA
Nanopore direct RNA sequencing (DRS) enables direct measurement of RNA molecules, including their native RNA modifications, without prior conversion to cDNA. However, commercial methods for molecular barcoding of multiple DRS samples are lacking, and community-driven efforts, such as DeePlexiCon, are not compatible with newer RNA chemistry flowcells and the latest-generation GPU cards. To overcome these limitations, we introduce SeqTagger, a rapid and robust method that can demultiplex direct RNA sequencing datasets with 99% precision and 95% recall.
View Article and Find Full Text PDFHow Much Data Are Enough? Toward Statistically Robust Adhesion Experiments by Atomic Force Microscopy.
Langmuir
January 2025
Department of Physical Chemistry II, University of Bayreuth, Universitaetsstrasse 30, Bayreuth 95447, Germany.
Direct force measurements by atomic force microscopy (AFM) have become an indispensable analytical tool in the last decades. Force measurements have been widely used for adhesion measurements, often in combination with the colloidal probe technique. For the latter technique, a colloidal particle is attached to the end of an AFM cantilever, proving great flexibility in terms of colloid/surface interaction to be studied.
View Article and Find Full Text PDFFacile preparation of iridium-based AIE polymer dots for sensitive electrochemiluminescence immunoassay of CD44 protein.
Anal Chim Acta
March 2025
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Department of Chemistry, Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address:
The development of aggregation-induced emission (AIE) luminophores is a fascinating and promising topic in electrochemiluminescence (ECL) bioanalysis. Herein, the AIE-active but water-insoluble [Ir(bt)₂(acac)] (bt = 2-phenylbenzothiazole, acac = acetylacetonate) was encapsulated within poly(styrene-maleic anhydride) (PSMA) using a simple nanoprecipitation method. This encapsulation strategy could effectively limit the free motion of Ir(bt)₂(acac) and trigger the aggregation-induced electrochemiluminescence (AIECL) effect.
View Article and Find Full Text PDFBehavioral evidence that repetitive responses in a Free-Movement Pattern Y-maze are associated with ageing-related deficit in working memory.
Behav Processes
January 2025
Surrey Sleep Research Centre, University of Surrey.
Visuospatial working memory (VSWM) is crucial for navigating complex environments and is known to decline with ageing. The Free-Movement Pattern (FMP) Y-maze, used in animal studies, provides a robust paradigm for assessing VSWM via analyses of individual differences in repeated alternating sequences of left (L) and right (R) responses (LRLR, etc.), the predominant search pattern in many species.
View Article and Find Full Text PDFGreen synthesis of a fluorescence optical probe for Fe based on Barberry derived carbon quantum dots: Deep insight on interaction from experimental and theoretical approach.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
A green facile method was developed to synthesize the carbon quantum dots from barberry, a native plant, as a new carbon source. The synthesis strategy is a simple one-step hydrothermal process without requiring hazardous chemical reagents. The spherical structure of b-CDs with an average particle size of 3.
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