AI Article Synopsis
Article Abstract
Background: In the recent years, the use of Doppler-echocardiography has become a standard non-invasive technique in the analysis of cardiac malformations in genetically modified mice. Therefore, normal values have to be established for the most commonly used inbred strains in whose genetic background those mutations are generated. Here we provide reference values for transthoracic echocardiography measurements in juvenile (3 weeks) and adult (8 weeks) 129/Sv mice.
Methods: Echocardiographic measurements were performed using B-mode, M-mode and Doppler-mode in 15 juvenile (3 weeks) and 15 adult (8 weeks) mice, during isoflurane anesthesia. M-mode measurements variability of left ventricle (LV) was determined.
Results: Several echocardiographic measurements significantly differ between juvenile and adult mice. Most of these measurements are related with cardiac dimensions. All B-mode measurements were different between juveniles and adults (higher in the adults), except for fractional area change (FAC). Ejection fraction (EF) and fractional shortening (FS), calculated from M-mode parameters, do not differ between juvenile and adult mice. Stroke volume (SV) and cardiac output (CO) were significantly different between juvenile and adult mice. SV was 31.93 ± 8.67 μl in juveniles vs 70.61 ± 24.66 μl in adults, ρ < 0.001. CO was 12.06 ± 4.05 ml/min in juveniles vs 29.71 ± 10.13 ml/min in adults, ρ < 0.001. No difference was found in mitral valve (MV) and tricuspid valve (TV) related parameters between juvenile and adult mice. It was demonstrated that variability of M-mode measurements of LV is minimal.
Conclusions: This study suggests that differences in cardiac dimensions, as wells as in pulmonary and aorta outflow parameters, were found between juvenile and adult mice. However, mitral and tricuspid inflow parameters seem to be similar between 3 weeks and 8 weeks mice. The reference values established in this study would contribute as a basis to future studies in post-natal cardiovascular development and diagnosing cardiovascular disorders in genetically modified mouse mutant lines.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651272 | PMC |
| http://dx.doi.org/10.1186/1476-7120-11-12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Alterations of Sensory-related Functional Brain Network Connectivity in Homozygous Knockout Mice.
Phenomics
October 2024
Human Phenome Institute, Institute of Medical Genetics and Genomics, MOE Key Laboratory of Contemporary Anthropology, Zhangjiang Fudan International Innovation Center, Fudan University, 825 Zhangheng Road, Shanghai, 201203 China.
Unlabelled: () is a neuro-specific gene linked to neurodevelopmental disorders and has recently been reported to function as a bidirectional emotional regulator, highlighting its molecular roles in the nervous system. However, the connections between , brain architecture, and functionality remain to be fully elucidated. Our study utilized 11.
View Article and Find Full Text PDFDo the provisional PRINTO Enthesitis/Spondylitis-Related JIA criteria capture youth with axial spondyloarthritis?
Arthritis Care Res (Hoboken)
December 2024
Gent University Hospital, Gent, Belgium; ZNA, Antwerp, Belgium.
Background: The Paediatric Rheumatology International Trials Organisation (PRINTO) recently undertook an effort to better harmonize the pediatric and adult arthritis criteria. These provisional criteria are being refined for optimal performance. We aimed to investigate differences between patients who did and did not fulfill these PRINTO criteria amongst youth diagnosed with juvenile spondyloarthritis (SpA) that met axial juvenile SpA (axJSpA) classification criteria.
View Article and Find Full Text PDFStone selection by wild chimpanzees shares patterns with Oldowan hominins.
J Hum Evol
December 2024
Department of Pedagogy, Chubu Gakuin University, Gifu, 504-0837, Japan; College of Life Science, Northwest University, Xi'an, 710069, China.
The use of broad tool repertoires to increase dietary flexibility through extractive foraging behaviors is shared by humans and their closest living relatives (chimpanzees, Pan troglodytes). However, comparisons between tool use in ancient human ancestors (hominins) and chimpanzees are limited by differences in their toolkits. One feature shared by primate and hominin toolkits is rock selection based on physical properties of the stones and the targets of foraging behaviors.
View Article and Find Full Text PDFThe magnetic resonance imaging (MRI) features of intracranial lesions in myelin oligodendrocyte glycoprotein-immunoglobulin G-associated disease (MOGAD).
Clin Radiol
November 2024
Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Aim: This study aimed to summarise and analyse the magnetic resonance imaging (MRI) characteristics of patients with myelin oligodendrocyte glycoprotein-immunoglobulin G-associated disease (MOGAD), and to enhance the accuracy of disease diagnosis and advance scientific research.
Materials And Methods: A retrospective collection of clinical data from 103 patients with MOGAD was conducted. The distribution and signal characteristics of intracranial lesions on MRI were analysed.
Dynamics of thermal tolerance plasticity across fish species and life stages.
J Therm Biol
December 2024
Department of Biology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, 7491, Norway; Department of Biology and Environmental Sciences, Kristineberg Center, University of Gothenburg, Fiskebäckskil, Sweden.
Climate warming with associated heat waves presents a concerning challenge for ectotherms such as fishes. During heatwaves, the ability to rapidly acclimate can be crucial for survival. However, surprisingly little is known about how different species and life stages vary in their acclimation dynamics, including the magnitude of change in thermal tolerance through acclimation (i.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!