Objective: To investigate the optimization system of SRAP-PCR molecular marker technology in the analysis on Pinellia ternata.
Method: SRAP-PCR reaction system for P. ternata was optimized by L16 (5(4)) orthogonal design with five elements (dNTPs, Mg2+, the template DNA, primers, Taq enzyme) and four standards.
Result: The most suitable forward primer for SRAP for Pinellia ternata was 5'-TGAGTCCAAACCGGAAG-3', while the reverse primer was 5'-GACTGCGTACGAATTACG-3'. The optimized reaction system contained 70 ng DNA template, 0.9 micromol x L(-1) primer, 0.20 mmol x L(-1) dNTP s, 1.5 - 2.0 mmol x L(-1) Mg2+, and 2 U Taq enzyme.
Conclusion: SRAP-PCR system for P. ternata is established to lay a foundation for future construction of SRAP genetic map of P. ternata.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Causes of Different Force-Velocity Relationships in Sprint Running Depending on Horizontal Loads and Profiling Methods.
Med Sci Sports Exerc
January 2025
National Institute of Fitness and Sports in Kanoya, Kanoya, Kagoshima, JAPAN.
Purpose: We aimed to clarify how the horizontal force-velocity (Fvh) relationship during over-ground sprint running differs with horizontal resistance loads and profiling methods (multiple- and single-trial methods).
Methods: Twelve males performed sprint running (one unresisted and five resisted) using a motorized loading device. During the trials, the ground reaction forces at every step were obtained using a 50 m force plate system.
Intracellular Delivery of Proteins by Protein-Recognizing Nanoparticles.
ACS Appl Mater Interfaces
January 2025
Department of Chemistry, Iowa State University, Ames, Iowa 50011-3111, United States.
Intracellular delivery of proteins can directly impact dysregulated and dysfunctional proteins and is a key step in the fast growing field of protein therapeutics. The vast majority of protein-delivery systems enter cells through endocytic pathways, but endosomal escape is a difficult and inefficient process, demanding fundamentally different methods of delivery. We report ultrasmall cationic molecularly imprinted nanoparticles that bind protein targets with high specificity through their uniquely distributed surface lysine groups.
View Article and Find Full Text PDFCatalytic Conversion of Carbon Dioxide to Long Chain Esters via Formate Hydroesterification.
Chemistry
January 2025
University of Missouri, Chemistry, 601 S. College Ave, 65211, Columbia, UNITED STATES OF AMERICA.
CO2-based hydroesterification is an attractive route to produce value added ester compounds, which could replace CO-based hydroesterification processes if sufficient catalytic technologies are developed. One path to CO2-based hydroesterification is through an organoformate intermediate, which is then used in olefin hydroesterification to generate the desirable esters. This route creates a net CO2-based hydroesterification process using tandem catalytic systems for CO2 hydrogenation to organoformate paired with formate-olefin hydroesterification.
View Article and Find Full Text PDFExpression Analysis of Cyst Specific Protein (CSP21) and Cellulose Synthase II (CSII) Genes in Acanthamoeba castellanii Trophozoites Exposed to Silver Nanoparticles Conjugated with Elaeagnus umbellata.
Acta Parasitol
January 2025
Department of Molecular Biology and Genetics, Ordu University, Ordu, Turkey.
Purpose: Acanthamoeba species are eucaryotic protozoa found predominantly in soil and water. They cause ulceration and vision loss in the cornea (Acanthamoeba keratitis) and central nervous system (CNS) infection involving the lungs (granulomatous amoebic encephalitis). Antiparasitic drugs currently used in the treatment of infections caused by Acanthamoeba species are not effective at the desired level in some anatomical regions such as the eye and CNS.
View Article and Find Full Text PDFRevolutionizing Methane Transformation with the Dual Production of Aromatics and Electricity in a Protonic Ceramic Electrocatalytic Membrane Reactor.
ACS Appl Mater Interfaces
January 2025
Department of Hydrogen and Electrochemistry, Idaho National Laboratory, Idaho Falls, ID 83415, United States.
Reducing the energy and carbon intensity of the conventional chemical processing industry can be achieved by electrochemically transforming natural gases into higher-value chemicals with higher efficiency and near-zero emissions. In this work, the direct conversion of methane to aromatics and electricity has been achieved in a protonic ceramic electrocatalytic membrane reactor through the integration of a proton-conducting membrane assembly and a trimetallic Pt-Cu/Mo/ZSM-5 catalyst for the nonoxidative methane dehydro-aromatization reaction. In this integrated system, a remarkable 15.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!