AI Article Synopsis
- The study aimed to find a novel method for identifying Astragali Radix by analyzing its DNA sequence, specifically the ITS region.
- Thirteen samples of Astragali Radix and six samples of various adulterants were tested using PCR to amplify the ITS sequence, followed by sequencing and comparative analysis through phylogenetic methods.
- The results showed that the ITS sequence can reliably differentiate Astragali Radix from its adulterants, proving it to be an effective molecular marker for their identification.
Article Abstract
Objective: To explore a new method for identification Astragali Radix from its adulterants by using ITS sequence.
Method: Thirteen samples of the different Astragali Radix materials and 6 samples of the adulterants of the roots of Hedysarum polybotrys, Medicago sativa and Althaea rosea were collected. ITS sequence was amplified by PCR and sequenced unidirectionally. The interspecific K-2-P distances of Astragali Radix and its adulterants were calculated, and NJ tree and UPGMA tree were constructed by MEGA 4.
Result: ITS sequences were obtained from 19 samples respectively, there were Astragali Radix 646-650 bp, H. polybotrys 664 bp, Medicago sativa 659 bp, Althaea rosea 728 bp, which were registered in the GenBank. Phylogeny trees reconstruction using NJ and UPGMA analysis based on ITS nucleotide sequences can effectively distinguish Astragali Radix from adulterants.
Conclusion: ITS sequence can be used to identify Astragali Radix from its adulterants successfully and is an efficient molecular marker for authentication of Astragali Radix and its adulterants.
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