AI Article Synopsis
- A phage display peptide library was employed to find mimotopes that mimic hepatitis A virus (HAV) antigenic determinants, showcasing the method's potential for epitope mapping.
- A purified anti-HAV monoclonal antibody was used to select positive clones from the library, followed by ELISA, competition assays, and DNA sequencing to confirm identity.
- Out of 10 positive clones, 9 shared a nucleotide sequence with HAV's VP1 protein, indicating they could serve as mimotopes for HAV, but no similarities were found with other HAV capsid proteins.
Article Abstract
Objective: A 12 mer phage display peptide library was used to identify hepatitis A virus mimotopes of antigenic determinants, to provide the feasibility of virus epitope mapping by using this approach.
Methods: Using purified anti-hepatitis A virus monoclonal antibody as affinity selective molecule, phage display peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
Results: 10 ELISA positive clones were chosen for DNA sequencing, and the displayed peptide sequences were deduced. 9 of them showed identical nucleotide sequence, and similarity in their amino acid sequence with VP1 of HAV HM175 was found, but no sequence homology was found between the other phage clone and the capsid proteins of HAV. Those peptides may behave as mimotopes of HAV.
Conclusion: The mimotope of HAV was selected by using phage display peptide library screening. The results provide the potential of this method to search for the mimotopes of the virus.
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