A bacteria-targeted MR contrast agent, Zn-, consisting of two Zn-dipicolylamine (Zn-dpa) groups conjugated to a Gd chelate has been synthesized and characterized. In vitro studies with and show that Zn- exhibits a significant improvement in bacteria labeling efficiency vs. control. Studies with a structural analogue, Zn-, indicate that removal of one Zn-dpa moiety dramatically reduces the agent's affinity for bacteria. The ability of Zn- to significantly reduce the of labeled vs. unlabeled bacteria, resulting in enhanced MR image contrast, demonstrates its potential for visualizing bacterial infections in vivo.
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