Polymerase chain reaction with insertion sequence-specific and -unrelated primers: a new tool for the identification of IS1548 insertion targets in Streptococcus agalactiae.

We developed a PCR method with outward insertion sequence-specific and -unrelated primers to identify IS1548 targets in the genome of unsequenced Streptococcus agalactiae strains. Our rapid and easy method allowed the identification of previously known but also of yet unnoticed integration sites in the three clinical isolates tested.