Using the seed- plate technique, we have isolated a strain ofF. proliferatum from rye grains that produces 3 fumonisins, fumonisin B1 (FB1), FB2 and FB3 on inoculated rice and corn. Inoculated corn and rice were extracted with an aqueous methanol solution and fumonisin concentrations estimated using high performance liquid chromatography. Production of all 3 fumonisins (FB1, FB2 and FB3) was much higher on rice than corn; ranging from 3816, 1068 and 985 ppm to 1643, 350 and 162 ppm respectively. We conclude that all natural substrates whereF. proliferatum is present as a component of the mycoflora should be monitored for the presence of fumonisins.
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http://dx.doi.org/10.1007/BF02945061 | DOI Listing |
Foods
January 2025
Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Deoxynivalenol (DON), fumonisin B (FB), and zearalenone (ZEN) are typical fusarium mycotoxins that occur worldwide in foodstuffs, posing significant health hazards to humans and animals. Single and combined exposure of DON, FB, and ZEN leads to intestinal toxicity but the toxicology mechanism research is still limited. In this study, we explored the cytotoxicity effects of DON, FB, ZEN, and their combination in rat intestinal epithelial cell line 6 (IEC-6) cells.
View Article and Find Full Text PDFToxins (Basel)
January 2025
U. S. National Poultry Research Center, Agriculture Research Service, U.S. Department of Agriculture, Athens, GA 30605, USA.
Fusarium mycotoxins often co-occur in broiler feed, and their presence negatively impacts health even at subclinical concentrations, so there is a need to identify the concentrations of these toxins that do not adversely affect chickens health and performance. The study was conducted to evaluate the least toxic effects of combined mycotoxins fumonisins (FUM), deoxynivalenol (DON), and zearalenone (ZEA) on the production performance, immune response, intestinal morphology, and nutrient digestibility of broiler chickens. A total of 960 one-day-old broilers were distributed into eight dietary treatments: T1 (Control); T2: 33.
View Article and Find Full Text PDFFront Microbiol
January 2025
Toxicology and Mycotoxin Research Unit, United States National Poultry Research Center, Agricultural Research Service, United States Department of Agriculture, Athens, GA, United States.
-a mycotoxigenic fungus and food safety threat-coinhabits maize kernels with . This protective endophyte produces secondary metabolites of interest, pyrrocidines A and B, which inhibit the growth of and specifically block fumonisin biosynthesis. Previous transcriptomic analyses found (FVEG_00314), a gene adjacent to the fumonisin biosynthetic gene cluster, to be induced over 4,000-fold in response to pyrrocidine challenge.
View Article and Find Full Text PDFFront Microbiol
January 2025
Laboratory of Food Microbiology and Mycotoxicology, Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Introduction: Microbial contamination remains a vital challenge across the food production chain, particularly due to mycotoxins-secondary metabolites produced by several genera of fungi such as , and . These toxins, including aflatoxins, fumonisins, ochratoxins, and trichothecenes (nivalenol, deoxynivalenol, T2, HT-2). These contaminants pose severe risks to human and animal health, with their potential to produce a variety of different toxic effects.
View Article and Find Full Text PDFFront Cell Infect Microbiol
January 2025
United States National Poultry Research Center, United States Department of Agriculture Toxicology and Mycotoxin Research Unit, Athens, GA, United States.
The mycotoxigenic fungi, and , commonly co-colonize maize in the field, yet their direct interactions at the chemical communication level have not been well characterized. Here, we examined if and how the two most infamous mycotoxins produced by these species, aflatoxin and fumonisin, respectively, govern interspecies growth and mycotoxin production. We showed that fumonisin producing strains of suppressed the growth of while non-producers did not.
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