AI Article Synopsis
- σ(E)-dependent cell lysis may play a crucial role in eliminating damaged cells during the stationary phase of Escherichia coli, contributing to long-term survival.
- Disruption of small regulatory RNA genes micA and rybB impacts the bacteria's ability to maintain a long-term stationary phase and leads to increased mutation rates.
- The study suggests that these processes help prevent excessive mutations by removing damaged cells, thereby aiding in the sustainability of bacterial populations over time.
Article Abstract
σ(E)-dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli. In order to explore the relationship of this process to long-term stationary phase existence, we considered that micA and rybB could be important small regulatory RNA (sRNA) genes for σ(E)-dependent cell lysis. A long-term stationary phase was observed at temperatures of <37°C, but not >38°C, and was found even in an rpoS knock-out background. Strains with disrupted micA or rybB were incapable of long-term stationary phase existence. Both strains drastically lost survivability accompanied by a dramatic accumulation of mutations. These findings allow us to speculate that σ(E)-dependent cell lysis plays a key role in the establishment of the long-term stationary phase, presumably by eliminating damaged cells and thus preventing the over-accumulation of mutations.
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| http://dx.doi.org/10.1159/000350370 | DOI Listing |
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