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Characterizing the heterogeneous contamination of commercial paper and board food packaging at different scales.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
UMR SayFood 0782, Université Paris-Saclay, INRAE, Palaiseau, AgroParisTech, France.
Assessing the contamination of paper and board (P&B) food packaging materials poses significant challenges due to the sensitivity limits of analytical methods and the low precision of sampling processes. This study aims to enhance the understanding of P&B food packaging contamination by investigating the distribution of contaminants at different scales using a combination of chromatographic and spectroscopic techniques. A total of 36 substances were targeted, including phthalates, photoinitiators, and bisphenol A.
View Article and Find Full Text PDFAnalysis of solution-phase biomolecular interactions by liquid chromatography: General strategies and recent developments.
J Pharm Biomed Anal
March 2025
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
The analysis of biomolecular interactions is important in characterizing and understanding many fundamental processes that occur in the body and biological systems. A variety of methods are available for studying the extent and rate of binding of these interactions. Some of these techniques are homogeneous methods, with all interacting components being present in the solution-phase, while others are heterogeneous, such as involving both solution-phase and solid-phase components.
View Article and Find Full Text PDFSingle-Cell Secretome Profiling Enabled by Selective Enrichment and NanoLC-MS/MS Analysis.
Angew Chem Int Ed Engl
January 2025
State Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Recent advances in single-cell proteomics enable the direct profiling of thousands of proteins from a single mammalian cell. However, due to the bottlenecks in detecting low-abundance secreted proteins and extracellular vesicle (EV) proteins (collectively referred to as the secretome) against a background of high-abundance proteins in serum-containing culture medium, the comprehensive investigation of the secretome at the single-cell level using nanoLC-MS/MS still remains challenging. Herein, we report a novel single-cell secretome profiling (SCSP) method by integrating the metabolic labeling of newly synthesized proteins, click chemistry-based enrichment, and in situ digestion of the labeled secretome in an alkyne-functionalized capillary micro-reactor, followed by nanoLC-MS/MS analysis.
View Article and Find Full Text PDFExploring the Correlation between LC-MS Multi-Attribute Method and Conventional Chromatographic Product Quality Assays through Multivariate Data Analysis.
AAPS J
November 2024
Global Vaccines and Biologics Commercialization, Merck & Co., Inc., 770 Sumneytown Pike, West Point, Pennsylvania, 19486, USA.
Biotherapeutics are subject to inherent heterogeneity due to the complex biomanufacturing processes. Numerous analytical techniques have been employed to identify, characterize, and monitor critical quality attributes (CQAs) to ensure product safety, and efficacy. Mass spectrometry (MS)-based multi-attribute method (MAM) has become increasingly popular in biopharmaceutical industry due to its potential to replace multiple traditional analytical methods.
View Article and Find Full Text PDFEvaluation of the atmospheric pollution by pesticides using lichens as biomonitors.
Sci Total Environ
December 2024
Institut Écocitoyen pour la Connaissance des Pollutions, Fos-sur-Mer, France.
The extensive use of pesticides combined with their persistence in the environment requires new methodologies to assess more effectively the population exposure to pesticides via air pollution. Biomonitoring pesticides with lichens has been poorly documented, although it represents a complementary approach to the usual active samplings, with an exposure to pesticides accumulated and integrated over several months. An optimized extraction procedure from the lichen Xanthoria parietina followed by a gas chromatographic-tandem mass spectrometric analysis is proposed here to quantify simultaneously 48 pesticides considered in France as priority active substances to monitor in the air.
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