Objective: To analyze the influence of food preparation methods on the composition of fatty acids and cholesterol in foods.
Methods: The chemical composition of cholesterol and fatty acids was analyzed in eight different types of meat and feijoada in relation to different methods of preparation.
Results: Feijoada, when prepared with the beans and meats in separate pots, has less cholesterol (12.1 vs. 16.1mg, respectively, p=0.005) and saturated fat (1.4 vs. 1.9mg, p=0.046) than when it is prepared in a single pot. Broiled chicken without the skin has less saturated fat when compared with skinless fried chicken (1,505 vs. 7,645mg, p=0.049). Broiled shrimp also has a lower saturated fat content than fried shrimp (532 vs. 1,262mg, p=0.049). Broiled ribeye steak without fat has a lower cholesterol content when compared with the fried steak (102 vs. 114mg, p=0.049).
Conclusion: The analysis indicates that the method of food preparation influences the fat content of foods, with potential impact on the prescription of low-fat and low-cholesterol diets.
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http://dx.doi.org/10.1016/j.ramb.2012.10.001 | DOI Listing |
Front Microbiol
January 2025
College Food Science and Light Industry, Nanjing Tech University, Nanjing, China.
A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.
View Article and Find Full Text PDFAnal Chim Acta
March 2025
The Radiology Department of Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, China. Electronic address:
Background: Real-time and rapid detection of ingredients in food has important significance for food safety. However, traditional detection methods not only require bulky and costly instruments but also are often based on single-mode analysis, limiting their accuracy and applications in point-of-care testing. Herein, an integrated and miniaturized dual-mode device based on colorimetric and photoacoustic (PA) principles is developed, using Au@Ag nanoparticles (Au@AgNPs) as signal probe and ascorbic acid (AA) and ascorbate oxidase (AAO) as analytes.
View Article and Find Full Text PDFProtein Expr Purif
January 2025
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China; National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital of Chinese Academy of Medical Sciences, Langfang Campus, Langfang, 065001, China. Electronic address:
As an important member of the Siglec family, SIGLEC-15 plays an important role in osteoclast differentiation, bone remodeling, and tumor immune evasion. In the tumor microenvironment, SIGLEC-15 functions independently of the B7-H1/PD-1 pathway. In this study, the SIGLEC-15 fusion protein (SIGLEC-15-Fc) was successfully expressed and purified using a eukaryotic expression system.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address:
Purple passion fruit peel (PPFP) is a common biomass waste. Meanwhile, hydrothermal carbonization (HTC) is a common technology used for thermal conversion of biomass waste. Herein, the aqueous phase (AP) of PPFP was determined using HTC, and its properties were studied.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Food Science and Engineering, Yangzhou University, Yang Zhou 225127, China. Electronic address:
Quercetin (Que) is a polyhydroxy flavonoid with strong inhibitory activity against cancer cells. However, the poor water solubility and low bioavailability of Que. limit its application in the functional food industry.
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