Transcripts of the bicistronic RNA3 of alfalfa mosaic virus were synthesized using the in vitro T7 run-off transcription system. Synthetic RNA3 containing one additional G nucleotide at the 5' end were found to be infectious when coinoculated with RNA1 and RNA2 and coat protein.
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Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.
Biotechnol J
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Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de Valencia-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
Virus-induced gene silencing (VIGS) represents a particularly relevant tool in agricultural species for studying gene functionality. This study presents a novel approach for utilizing viruses belonging to the 30K family of movement proteins (MPs) as VIGS vectors. The method described here employs smaller inserts (54 bp or less) than those commonly used (100-500 bp).
View Article and Find Full Text PDFOccurrence of Snake River alfalfa virus in alfalfa () in Oregon and in Northern California.
Plant Dis
December 2024
University of Idaho, EPPN, 875 Perimeter Dr., MS 2329, Moscow, Idaho, United States, 83844-2329;
Alfalfa (Medicago sativa L.) is a commonly grown forage crop in Oregon and California harvested on 350,000 and 480,000 acres, respectively, in 2023 (USDA-NASS 2023). Forage alfalfa is grown as a perennial crop for about four years in the same field and each season, the crop is cut 3-4 times for hay production.
View Article and Find Full Text PDFIdentification of Host Factors Interacting with Movement Proteins of the 30K Family in .
Int J Mol Sci
November 2024
Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de Valencia-CISC, 46022 Valencia, Spain.
Article Synopsis
- The study focuses on how viral proteins, specifically movement proteins (MPs) of the 30K family, interact with host factors in plants, which is key to viral infection.
- Researchers used the alfalfa mosaic virus (AMV) MP model, modifying its coding sequence with tags to successfully identify 121 potential host interactors.
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BMC Plant Biol
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View Article and Find Full Text PDFFirst detection of alfalfa mosaic virus in in Chile.
Plant Dis
November 2024
Pontificia Universidad Catolica de Chile, Departamento de Ciencias Vegetales, Facultad de Agronomía y Sistemas Naturales, Santiago, RM, Chile;
Article Synopsis
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