AI Article Synopsis
- The study assessed a new sodC-based RT-PCR test for detecting Neisseria meningitidis in cerebrospinal fluid and serum, highlighting its ability to identify nongroupable strains commonly found in asymptomatic carriers.
- However, the sodC test showed 7.5% lower sensitivity compared to the traditional ctrA method.
- Due to the risk of false-negative results with the sodC-only approach, caution is advised when using it for diagnosing meningococcal meningitis.
Article Abstract
We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970655 | PMC |
| http://dx.doi.org/10.1590/0074-0276108022013020 | DOI Listing |
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Use of sodC versus ctrA for real-time polymerase chain reaction-based detection of Neisseria meningitidis in sterile body fluids.
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Article Synopsis
- The study assessed a new sodC-based RT-PCR test for detecting Neisseria meningitidis in cerebrospinal fluid and serum, highlighting its ability to identify nongroupable strains commonly found in asymptomatic carriers.
- However, the sodC test showed 7.5% lower sensitivity compared to the traditional ctrA method.
- Due to the risk of false-negative results with the sodC-only approach, caution is advised when using it for diagnosing meningococcal meningitis.
sodC-based real-time PCR for detection of Neisseria meningitidis.
PLoS One
May 2011
Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Real-time PCR (rt-PCR) is a widely used molecular method for detection of Neisseria meningitidis (Nm). Several rt-PCR assays for Nm target the capsule transport gene, ctrA. However, over 16% of meningococcal carriage isolates lack ctrA, rendering this target gene ineffective at identification of this sub-population of meningococcal isolates.
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