Gan-Lu-Yin Inhibits Proliferation and Migration of Murine WEHI-3 Leukemia Cells and Tumor Growth in BALB/C Allograft Tumor Model.

Evid Based Complement Alternat Med

School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Department of Pharmacy, Da Chien General Hospital, Miaoli 36052, Taiwan.

Published: July 2013

AI Article Synopsis

  • The study examined Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, and its antitumor effects on leukemia, specifically using WEHI-3 leukemic cells and a BALB/c mice model.
  • GLY significantly reduced cell proliferation and migration, induced differentiation of WEHI-3 cells, and caused cell cycle arrest in the G1 phase while impacting key signaling proteins such as FAK, Akt, and ERK.
  • The formula also demonstrated a decrease in leukemic infiltration in the liver and spleen of mice, suggesting its potential as a promising treatment for leukemia in clinical settings.

Article Abstract

The aim of this study was to explore the antitumor effect of Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, on leukemia. Ethanolic extract of GLY was applied to evaluate its regulatory mechanisms in proliferation, migration, and differentiation of WEHI-3 leukemic cells as well as antitumor effect on BALB/c mice model. The results showed that GLY markedly reduced cell proliferation and migration with induced differentiation of WEHI-3 cells. The expression level of phosphorylated FAK, Akt, ERK1/2, and Rb was decreased p21 expression while level was increased in WEHI-3 treated with GLY. The results of cell cycle analysis revealed that GLY treatment could markedly induce G1 phase arrest and decrease cell population in S phase. Moreover, experimental results demonstrated that GLY decreased the protein expression and enzyme activity of MMP-2 and MMP-9. GLY treatment also reduced WEHI-3 leukemic infiltration in liver and spleen and tumor growth in animal model. Accordingly, GLY demonstrated an inhibitory effect on tumor growth with a regulatory mechanism partially through inhibiting FAK, Akt, and ERK expression in WEHI-3 cells. GLY may provide a promising antileukemic approach in the clinical application.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613066PMC
http://dx.doi.org/10.1155/2013/684071DOI Listing

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