Squid tubes were packed with 100% (w/w of squid) dry ice (PI), 20% dry ice and 50% water ice (PII) and 50% water ice (PIII) in polyethylene bags and store in thermocole boxes at room temperature (32 ± 2°C) for 24 h. Quality changes curing storage were studied. Lowest temperature of -30.3°C was attained in PI while it was 15-16°C in PII and PIII at 1 h of storage. The gas compositions in packages initially were 21% O2, 0.4% CO2 and 78.1% N2 in PI, PII and PIII, respectively. During storage for 24 h highest level of 82.5% CO2 was noticed in PII. Fresh squid tubes had bacterial flora of Hafnia, Pseudomonas, Bacillus, Flavobacterium and Alcaligens. Hafnia constituted 74% of the flora. Alcaligenes (47%), Alteromonas (30%) and Alcaligenes (56%) were dominant in squid tubes stored in 100% dry ice, in the combination package, and in 100% water ice, respectively. Increase in total volatile base nitrogen and trimethylamine nitrogen, no definite trend in free fatty acid values in all packages while increase in pH in PI and PIII and no consistent changes in PI were noticed during storage for 24 h. The PI had lowest bacterial counts and PIII the highest. Squids stored in PI and PII were sensorily acceptable after 24 and 18 h, respectively.
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http://dx.doi.org/10.1007/s13197-010-0066-5 | DOI Listing |
Plant Methods
December 2024
Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences, Ohwashi 1-1, Tsukuba, Ibaraki, Japan.
Background: Gene expression is a fundamental process for plants to express their phenotype, and its analysis is the basis of molecular studies. However, the instability of RNA often poses an obstacle to analyzing plants grown in fields or remote locations where the availability of liquid nitrogen or dry ice is limited. To deepen our understanding of plant phenotypes and tolerance to field-specific stresses, it is crucial to develop methodologies to maintain plant RNA intact and safely transfer it for downstream analyses such as qPCR and RNA-seq.
View Article and Find Full Text PDFSci Rep
December 2024
Physical Geography, Institute of Geography, Technical University Dresden, Dresden, Germany.
It is generally accepted that a weakening of the North Atlantic thermohaline circulation caused the Younger Dryas cooling. Although the role of seasonality was emphasized previously, this aspect is rarely considered yet, and it remains elusive how this impacted hydroclimate during winters and summers across Central Europe. Here, we coupled biomarker-based δO and δH from Bergsee in southern Germany to reconstruct deuterium excess as a proxy for evaporation history from the Bølling-Allerød to the Preboreal.
View Article and Find Full Text PDFInfluenza Other Respir Viruses
December 2024
Laboratory for Drug Discovery and Disease Research, Shionogi & Co, Ltd, Osaka, Japan.
Background: Evaluation of infectious virus titer is a challenge for respiratory syncytial virus (RSV) clinical trials because of the labile nature of RSV and rapid loss of infectivity without proper specimen handling. However, there has been no rigorous investigation into RSV stability in clinical specimens.
Methods: RSV stability was investigated by evaluating virus titers and defined as titer variation from baseline within three standard deviations of our titration assay.
Rapid Commun Mass Spectrom
February 2025
School of Earth, Environment & Society, McMaster University, Hamilton, ON, Canada.
Rationale: The stable isotope compositions of atmospheric CO can provide useful insight into various geochemical processes and carbon cycles on Earth, which is critical for understanding of Earth's changing climate. Here, we present a simple and cost-effective analytical method for the collection and measurement of carbon and oxygen isotope compositions of atmospheric CO.
Methods: Air samples of ~150 mL were collected individually or collectively using our simple active air collection system and then extracted on a vacuum purification line to remove noncondensable gases and atmospheric water vapor.
Glob Chang Biol
December 2024
Museo Nacional de Ciencias Naturales, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Global change is causing the melting of ice masses, permafrost thawing, and the shrinking of glaciers, thereby reshaping nature's rhythms. Longer thaw phases and more frequent dry periods are transforming water-driven transitional ecosystems (e.g.
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