We describe a robust method for the isolation of pure, intact RNA suitable for transcriptome studies from mycobacteria with consistent yields of 1 μg to 3 μg total RNA per 10(7) cells. The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA.
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| http://dx.doi.org/10.1016/j.mimet.2013.03.018 | DOI Listing |
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