AI Article Synopsis
- The study aimed to create and evaluate a mouse model of epithelial ovarian cancer that can be used to mimic human disease in immunocompetent hosts.
- ID8 mouse ovarian cells were implanted into C57BL/6 mice, and over 16 weeks, researchers monitored tumor growth, spread, and similar conditions using various imaging and analysis techniques.
- Results showed significant tumor growth, with ascites and metastasis appearing by week 12, and the model mirrored human ovarian cancer traits, although there was less blood vessel formation compared to human tumors.
Article Abstract
Background/aim: To develop and characterize the pre-clinical suitability of a syngeneic mouse epithelial ovarian cancer model in immunocompetent hosts.
Materials And Methods: ID8 mouse ovarian surface epithelium cells were implanted into the left ovarian bursa of C57BL/6 mice. Using conventional as well as ultrasound-based techniques and histopathological analysis, the tumor weights, volumes, metastases, ascites and vascularity were observed over a period of 16 weeks.
Results: Ovarian weights and volume increased 12- and 7-fold, respectively. Ultrasound measurements of ovarian ID8 tumors correlated with the actual size obtained following surgical excision. Ascites and metastasis were first observed at 12 weeks post-orthotopic implantation. Histopathological analysis indicated similarities between orthotopically-generated ovarian tumors and human ovarian tumors. However, there was less evidence of angiogenesis in this animal model.
Conclusion: The development of this mouse model closely replicates characteristics seen in human ovarian cancer with feasibility of using ultrasound to assess tumor formation, progression and vascularization.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!