AI Article Synopsis
Article Abstract
The high accumulation of a recombinant protein in rice endosperm causes endoplasmic reticulum (ER) stress and in turn dramatically affects endogenous storage protein expression, protein body morphology and seed phenotype. To elucidate the molecular mechanisms underlying these changes in transgenic rice seeds, we analyzed the expression profiles of endogenous storage proteins, ER stress-related and programmed cell death (PCD)-related genes in transgenic lines with different levels of Oryza sativa recombinant alpha antitrypsin (OsrAAT) expression. The results indicated that OsrAAT expression induced the ER stress and that the strength of the ER stress was dependent on OsrAAT expression levels. It in turn induced upregulation of the expression of the ER stress response genes and downregulation of the expression of the endogenous storage protein genes in rice endosperm. Further experiments showed that the ER stress response upregulated the expression of PCD-related genes to disturb the rice endosperm development and induced pre-mature PCD. As consequence, it resulted in decrease of grain weight and size. The mechanisms for the detriment seed phenotype in transgenic lines with high accumulation of the recombinant protein were elucidated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11103-013-0056-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Imaging Lipidomics and Metallomics of Brown Rice Cultivars Used for Sake Production.
Mass Spectrom (Tokyo)
December 2024
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Many previous studies have reported various phospholipids and elements that affect sake production; however, it seems to be challenging to investigate individual types in each rice variety due to their high diversity, not to mention their distribution patterns. Since its introduction, mass spectrometry imaging (MSI) has gained attention in various fields as a simple compound visualization technique. The current study highlights the progress of powerful MSI in comprehensively analyzing phospholipids and minerals in brown rice for sake production.
View Article and Find Full Text PDFGenomic regions associated with flag leaf and panicle architecture in rice (Oryza sativa L.).
BMC Genomics
December 2024
Texas A&M AgriLife Research Center, Beaumont, TX, 77713, USA.
Background: Flag leaf (FL) and panicle architecture (PA) are critical for increasing rice grain yield as well as production. A genome-wide association study (GWAS) can better understand the genetic pathways behind complex traits like FL and PA.
Results: In this study, 208 diverse rice germplasms were grown in the field at the Texas A&M AgriLife Research Center at Beaumont, TX, during 2022 and 2023 following Augmented Randomized Complete Block Design.
Hydrological transport and endosperm weakening mechanisms during dormancy release in Tilia henryana seeds.
J Plant Physiol
December 2024
College of Forestry, Nanjing Forestry University, 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu, 210037, PR China; Co-innovation Center for Sustainable Forestry in Southern China, Southern Tree Inspection Center National Forestry Administration, 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu, 210037, PR China. Electronic address:
Seed germination is a pivotal stage in the plant life cycle, with endosperm weakening and radicle elongation serving as crucial prerequisites for successful endospermic seed germination. Tilia henryana seeds exhibit deep dormancy, necessitating a period of 2-3 years to germinate in a natural environment, and the germination rate is extremely low. This study employed morphological and physiological approaches to dynamically analyzing the hydrological mechanism and the endosperm weakening process during the dormancy release of T.
View Article and Find Full Text PDFAn Integrative Analysis of the Transcriptome and Proteome of Rice Grain Chalkiness Formation Under High Temperature.
Plants (Basel)
November 2024
State Key Laboratory of Rice Biology, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.
Exposure to high temperatures can impair the grain-filling process in rice ( L.), potentially leading to the formation of chalky endosperm, but the molecular regulation mechanism remains largely elusive. Here, we reported that high-temperature (HT) stress (day/night, 35 °C/30 °C) reduces both the grain-filling rate and grain weight of Ningjing 1 variety compared to normal temperatures (NT, day/night, 28 °C/23 °C).
View Article and Find Full Text PDFCooked germ-remained milled rice presents high stability in water-holding capacity and textural properties during frozen storage-The protection effect of oil-phase reabsorption.
Food Chem
December 2024
School of Food Science and Technology, Jiangnan University, Lihu Avenue 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Avenue 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Avenue 1800, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Lihu Avenue 1800, Wuxi 214122, China. Electronic address:
To enhance the stability in textural attributes of frozen cooked rice, this study has found and proposed a new strategy: retaining the rice germ in milling process. Each rice germ is comparable to a native lipid-rich microcapsule attached to the endosperm part. In the gelatinization process, leached germ oil was reabsorbed in the outer layer of rice granule.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!