AI Article Synopsis
- Researchers created star-shaped polymers made from polyethylene glycol (PEG) to deliver nucleic acids (NA) for potential use in gene therapy.
- These polymers were made using a method called atom transfer radical polymerization (ATRP) and were characterized for their structure and properties.
- When combined with plasmid DNA (pDNA) or short interfering RNA (siRNA), the polymers formed effective delivery systems that worked well even at low amounts, showing the significance of releasing the nucleic acids for successful delivery.
Article Abstract
Poly(ethylene glycol) (PEG)-based star polymers with a cationic core were prepared by atom transfer radical polymerization (ATRP) for in vitro nucleic acid (NA) delivery. The star polymers were synthesized by ATRP of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and ethylene glycol dimethacrylate (EGDMA). Star polymers were characterized by gel permeation chromatography, zeta potential, and dynamic light scattering. These star polymers were combined with either plasmid DNA (pDNA) or short interfering RNA (siRNA) duplexes to form polyplexes for intracellular delivery. These polyplexes with either siRNA or pDNA were highly effective in NA delivery, particularly at relatively low star polymer weight or molar ratios, highlighting the importance of NA release in efficient delivery systems.
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| http://dx.doi.org/10.1021/bm4003199 | DOI Listing |
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