AI Article Synopsis
- The article presents a new microchip that allows for real-time imaging and applying mechanical stress to monolayered cell cultures.
- The setup includes an 8-µm thick membrane and a programmable pneumatic system to control stretching experiments with different cell lines.
- Results show that the chip can deliver a maximum strain of 69%, revealing significant morphological changes in cells, making it a valuable tool for researching tissue regeneration and cell growth conditions.
Article Abstract
Aim: This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures.
Materials & Methods: Cells were cultured on an 8-µm thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass coverslip. A programmable pneumatic control system was developed to actuate this platform. Multiple stretching experiments were conducted with various cell lines.
Results: The platform provides a maximum uniform strain of 69%. Acute and long-term cell morphological changes were observed. The supreme imaging capability was verified by real-time imaging of transfected COS-7 stretching and poststretching imaging of immunofluorescence-stained PTK2.
Conclusion: The platform reported here is a powerful tool for studying mechanically induced physiological changes in cells. Such a device could be used in tissue regeneration for maintaining essential cell growth conditions.
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| http://dx.doi.org/10.2217/nnm.13.45 | DOI Listing |
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