AI Article Synopsis
- A combined optogenetic-electrophysiological approach was used to identify which entorhinal cells connect and influence place cells in the hippocampus.
- Channelrhodopsin-2 (ChR2) was selectively introduced into specific neurons targeting the hippocampus to analyze their behavior in response to light.
- Responsive cells included primarily grid cells, but also border cells, head-direction cells, and others, indicating that place fields might be formed from various types of entorhinal cells working together.
Article Abstract
We used a combined optogenetic-electrophysiological strategy to determine the functional identity of entorhinal cells with output to the place-cell population in the hippocampus. Channelrhodopsin-2 (ChR2) was expressed selectively in the hippocampus-targeting subset of entorhinal projection neurons by infusing retrogradely transportable ChR2-coding recombinant adeno-associated virus in the hippocampus. Virally transduced ChR2-expressing cells were identified in medial entorhinal cortex as cells that fired at fixed minimal latencies in response to local flashes of light. A large number of responsive cells were grid cells, but short-latency firing was also induced in border cells and head-direction cells, as well as cells with irregular or nonspatial firing correlates, which suggests that place fields may be generated by convergence of signals from a broad spectrum of entorhinal functional cell types.
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| http://dx.doi.org/10.1126/science.1232627 | DOI Listing |
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