AI Article Synopsis
- DNMTs (DNA methyltransferases) play a key role in DNA methylation and are dysregulated in HCV-related liver cancer.
- Advances in research using shRNA interference identify DNMT1 and DNMT3B as important for HCV propagation, as their down-regulation hinders infection in liver cells.
- DNMT inhibitors like 5-Aza-C and 5-Aza-dC effectively reduce HCV infection and replication, indicating that targeting DNMTs could be a promising strategy for HCV treatment.
Article Abstract
DNA methyltransferases (DNMTs) are responsible for establishing and maintaining DNA methylation, which are dysregulated in hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC). In this report, using lentivirus-mediated shRNA interference technology, we identified DNMT1 and DNMT3B as host factors involved in HCV propagation. Our results demonstrated that down-regulation of DNMT1 or DNMT3B expression in Huh7.5.1 cells severely impaired cell culture-produced HCV (HCVcc) infection. Furthermore, knockdown of DNMT1 or DNMT3B did not affect HCV entry and internal ribosome entry site (IRES)-directed translation but did inhibit subgenomic replication. In contrast, knockdown of DNMT3A had no significant effect on HCV infection, entry, translation, or replication, which suggested that DNMT3A did not play a significant role in HCV life cycle. Moreover, we showed that DNMT inhibitors 5-Aza-C and 5-Aza-dC significantly suppressed HCVcc infection, viral RNA replication, and protein expression. These results suggest that DNMTs are critical for HCV replication and may represent potent targets for the treatment of HCV infection.
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| http://dx.doi.org/10.1016/j.virol.2013.03.005 | DOI Listing |
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