AI Article Synopsis
- 2-Aminopurine (2AP) is a fluorescent probe used to investigate dynamics and energy transfer in DNA/RNA, but its fluorescence behavior varies across solvents.
- Researchers explored the excited-state dynamics of 2-aminopurine 2'-deoxyriboside (2APdr) using various spectroscopic methods in different solvents (acetonitrile, ethanol, aqueous buffer at pH 7).
- Findings revealed that up to 40% of the initially excited 2APdr population transitions to a triplet state instead of emitting fluorescence, with this rate influenced by the solvent's hydrogen-donor ability and polarity.
Article Abstract
2-Aminopurine is a fluorescent probe widely used to study local dynamics as well as charge and energy transfer reactions in DNA/RNA. Despite its broad utilization, the nonradiative relaxation pathways responsible for the variation in its fluorescence quantum yield and fluorescence lifetime in different solvents are still under scrutiny. In this work we use steady-state absorption and emission spectroscopy and broad-band transient absorption covering the time scale from femtoseconds to microseconds to investigate the excited-state dynamics of 2-aminopurine 2'-deoxyriboside (2APdr) in acetonitrile, ethanol, and aqueous buffer solution at pH 7. It is shown that up to ~40% of the initial excited-state population decays by intersystem crossing to the triplet state depending on the solvent used, thus competing effectively with fluorescence emission. Furthermore, the rate of formation and yield of the triplet state depend sensitively on the hydrogen-donor ability and polarity of the solvent.
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| http://dx.doi.org/10.1039/c3pp25437b | DOI Listing |
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