AI Article Synopsis
- The study explores how nano-sized hydroxyapatite (nano-HA) enhances the expression of bone morphogenetic protein (BMP)-2 in human periodontal ligament (PDL) cells, which is crucial for periodontal regeneration.
- Using real-time PCR and immunofluorescence, researchers found that BMP-2 levels increased in a dose- and time-dependent manner when stimulated with nano-HA.
- The mechanism involves the p38 MAP kinase pathway, with results showing that inhibiting this pathway decreased BMP-2 expression, indicating the importance of nano-HA in future periodontal treatments.
Article Abstract
Objective: Bone morphogenetic protein (BMP)-2 promotes the osteoblastic differentiation of human periodontal ligament (PDL) cells, which play a pivotal role in periodontal regeneration. Recently, nano-sized hydroxyapatite (nano-HA) has been highlighted due to its advantageous features over micro-sized materials.
Design And Results: We investigated the effect of nano-HA on BMP-2 expression in human PDL cells. Real time PCR analysis revealed that the expression of BMP-2 increased upon stimulation with nano-HA in dose- and time-dependent manners. An immunofluorescence assay demonstrated the synthesis of BMP-2 proteins. Concentrations of Ca(2+) as well as phosphate (Pi) in culture supernatants were unchanged, suggesting that nano-HA functioned as a nanoparticle rather than as a possible source for releasing Ca(2+) and/or Pi extracellularly, which were shown to also enhance the expression of BMP-2. Nano-HA-induced BMP-2 expression was dependent on the p38 MAP kinase pathway because increases in BMP-2 expression were inhibited by treatment with SB203580, a p38 inhibitor, and phosphorylation of p38 was detected by Western blotting.
Conclusions: This novel mechanism of nano-HA will be important for the rational design of future periodontal regeneration.
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| http://dx.doi.org/10.1016/j.archoralbio.2013.02.014 | DOI Listing |
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