Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Trueperella pyogenes DNA based on the plo gene sequence that encodes pyolysin. The results showed that target DNA was amplified specifically and with a detection limit 100-fold greater than PCR.
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| http://dx.doi.org/10.1016/j.mimet.2013.03.002 | DOI Listing |
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