AI Article Synopsis
- Autofluorescence spectroscopy is a technique that allows for detailed analysis of biological samples without the need for labeling, but has been limited to visible light and near-infrared due to a lack of appropriate tools.
- A new synchrotron-coupled deep UV (DUV) microspectrofluorimeter developed in 2010 expands the capabilities by enabling the use of naturally occurring fluorophores excited in the DUV range.
- This advancement demonstrates the usefulness of DUV autofluorescence in studying tissue histology and cell biology across various biological samples, from cultured cells to plant stems.
Article Abstract
Background Information: Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interesting and naturally occurring fluorophores can be excited in the UV and deep UV (DUV), but cannot be monitored in cellulo nor in vivo due to a lack of available microscopic instruments working in this wavelength range. To fulfil this need, we have developed a synchrotron-coupled DUV microspectrofluorimeter which is operational since 2010. An extended selection of endogenous autofluorescent probes that can be excited in DUV, including their spectral characteristics, is presented. The distribution of the probes in various biological samples, including cultured cells, soft tissues, bone sections and maize stems, is shown to illustrate the possibilities offered by this system. In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology.
Results: To fulfil this need, we have developed a synchrotron-coupled DUV microspectrofluorimeter which is operational since 2010. An extended selection of endogenous autofluorescent probes that can be excited in DUV, including their spectral characteristics, is presented. The distribution of the probes in various biological samples, including cultured cells, soft tissues, bone sections and maize stems, is shown to illustrate the possibilities offered by this system. In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology.
Conclusions: In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology.
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| http://dx.doi.org/10.1111/boc.201200075 | DOI Listing |
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