Performance of AFEX™ pretreated rice straw as source of fermentable sugars: the influence of particle size.

Background: It is widely believed that reducing the lignocellulosic biomass particle size would improve the biomass digestibility by increasing the total surface area and eliminating mass and heat transfer limitation during hydrolysis reactions. However, past studies demonstrate that particle size influences biomass digestibility to a limited extent. Thus, this paper studies the effect of particle size (milled: 2 mm, 5 mm, cut: 2 cm and 5 cm) on rice straw conversion. Two different Ammonia Fiber Expansion (AFEX) pretreament conditions, AFEX C1 (low severity) and AFEX C2 (high severity) are used to pretreat the rice straw (named as AC1RS and AC2RS substrates respectively) at different particle size.

Results: Hydrolysis of AC1RS substrates showed declining sugar conversion trends as the size of milled and cut substrates increased. Hydrolysis of AC2RS substrates demonstrated opposite conversion trends between milled and cut substrates. Increasing the glucan loading to 6% during hydrolysis reduced the sugar conversions significantly in most of AC1RS and AC2RS except for AC1RS-2 mm and AC2RS-5 cm. Both AC1RS-2 mm and AC2RS-5 cm indicated gradual decreasing trends in sugar conversion at high glucan loading. Analysis of SEM imaging for URS and AFEX pretreated rice straw also indicated qualitative agreement with the experimental data of hydrolysis. The largest particle size, AC2RS-5 cm produced the highest sugar yield of 486.12 g/kg of rice straw during hydrolysis at 6% glucan loading equivalent to 76.0% of total theoretical maximum sugar yield, with an average conversion of 85.9% from total glucan and xylan. In contrast, AC1RS-5 cm gave the lowest sugar yield with only 107.6 g/kg of rice straw, about 16.8% of total theoretical maximum sugar yield, and equivalent to one-quarter of AC2RS-5 cm sugar yield.

Conclusions: The larger cut rice straw particles (5 cm) significantly demonstrated higher sugar conversion when compared to small particles during enzymatic hydrolysis when treated using high severity AFEX conditions. Analysis of SEM imaging positively supported the interpretation of the experimental hydrolysis trend and kinetic data.