AI Article Synopsis
- Nigella sativa (black cumin) has demonstrated significant potential in inhibiting the growth of human cervical cancer cells, specifically SiHa, with a notable 88.3% reduction in cell proliferation observed at a concentration of 125 µL/mL.
- The study found that Nigella sativa extract activates key proteins involved in apoptosis, specifically increasing the expression of caspases-3, -8, and -9.
- Overall, the research suggests that Nigella sativa could serve as a promising alternative treatment option for cervical cancer by promoting cell death through a mechanism involving p53 and caspase activation.
Article Abstract
Nigella sativa (NS), also known as black cumin, has long been used in traditional medicine for treating various cancer conditions. In this study, we sought to investigate the potential anti-cancer effects of NS extract using SiHa human cervical cancer cells. NS showed an 88.3% inhibition of proliferation of SiHa human cervical cancer cells at a concentration of 125 microL/mL methanolic extract at 24 h, and an IC50 value 93.2 microL/mL. NS exposure increased the expression of caspase-3, -8 and -9 several-fold. The analysis of apoptosis by Dead End terminal transferase-mediated dUTP-digoxigenin end labeling (TUNEL) assay was used to further confirm that NS induced apoptosis. Thus, NS was concluded to induce apoptosis in SiHa cell through both p53 and caspases activation. NS could potentially be an alternative source of medicine for cervical cancer therapy.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!