A new amide glycoside, 3-alpha-glucopyranosyloxy-N-methyl-3-phenylpropanamide (1), and a new cyclodipeptide, (3S,6S)-3,6-bis(4-acetamidobutyl)piperazine-2,5-dione (2), were isolated from the solid culture of Streptomycetes michiganensis strain SC0642. Their structures were elucidated by spectroscopic methods.
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ACS Infect Dis
December 2024
Department of Chemistry, University of Waterloo, 200 University Ave. West, Waterloo, Ontario N2L3G1, Canada.
The calcium-dependent antibiotics (CDAs) are a group of seven closely related membrane-active cyclic lipopeptide antibiotics (cLPAs) first isolated in the early 1980s from the fermentation broth of . Their target was unknown, and the mechanism of action is uncertain. Herein, we report new routes for the synthesis of CDA4b and its analogues, explore the structure-activity relationships at its lipid tail and at positions 3, 9, and 11, and determine the CDAs' lipid target.
View Article and Find Full Text PDFInt J Mol Sci
October 2024
King Abdullah International Medical Research Center, King Saud Bin Abdelaziz University for Health Sciences, Jeddah 21423, Saudi Arabia.
Geldanamycin, an -macrolide composed of a rigid benzoquinone ring and an aliphatic -bridge, was isolated from . Geldanamycin is a potent heat shock protein inhibitor with remarkable antiproliferative activity. However, it shows pronounced hepatotoxicity in animal models and unfavorable pharmacokinetic properties.
View Article and Find Full Text PDFInt J Mol Sci
October 2024
Grupo de Investigación en Simulación, Diseño, Control y Optimización de Procesos (SIDCOP), Departamento de Ingeniería Química, Universidad de Antioquia, Medellín 050010, Colombia.
is a species used worldwide to industrially produce clavulanic acid (CA), a molecule that enhances antibiotic effectiveness against β-lactamase-producing bacterial strains. Despite its low inherent CA production, hyper-producing strains have been developed. However, genomic analyses specific to and CA biosynthesis are limited.
View Article and Find Full Text PDFFront Microbiol
September 2024
Laboratorio de Ecología Molecular y Microbiología Aplicada, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad Católica del Norte, Antofagasta, Chile.
Thermophilic microorganisms possess several adaptations to thrive in high temperature, which is reflected as biosynthesis of proteins and thermostable molecules, isolation and culture represent a great methodological challenge, therefore High throughput sequencing enables screening of the whole bacterial genome for functional potential, providing rapid and cost-effective information to guide targeted cultures for the identification and characterization of novel natural products. In this study, we isolated two thermophilic bacterial strains corresponding to LB7 and LB8, from the microbial mats in the Atacama Desert. By combining genome mining, targeted cultures and biochemical characterization, we aimed to identify their capacity to synthesize bioactive compounds with antimicrobial properties.
View Article and Find Full Text PDFCancers (Basel)
September 2024
Department of Radiation Medicine, James M. Slater, MD Proton Treatment & Research Center, Loma Linda University Health, Loma Linda, CA 92350, USA.
The duocarmycin family is a group of potent cytotoxic agents originally isolated from the bacterium Streptomyces. This discovery has spurred significant interest due to duocarmycins' unique chemical structures and powerful mechanism of action. This review comprehensively details the history of the duocarmycin family, the current understanding of their therapeutic potential, and the major clinical trials that have been conducted.
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