Development of an electrochemical DNA biosensor for direct detection and discrimination of double-stranded plasmid (ds-Pl) without the need for denaturation of the target plasmid sample using a peptide nucleic acid (PNA) oligomer as the probe is described. This goal was achieved by modification of gold electrode with 6-mercapto-1-hexanol following monolayer self-assembly of cysteine conjugated 20-mer PNA oligomer probe, complementary to the HCV core/E1 region, which binds to ds-Pl and forms PNA/ds-Pl structure. The significant variation in differential pulse voltammetric response of methylene blue on the probe modified electrode upon contacting with complementary double-strand plasmid to form PNA/ds-Pl triplex structure is the principle of target plasmid detection. The results indicated that the reduction peak current was linear with the concentration of complementary strand in the range of 10-300 pg/μl with a detection limit of 9.5 pg/μl.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2013.01.063 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolic syndrome index measurement tool (MSI): scale development, reliability and validity study.
BMC Public Health
January 2025
Vocational School of Health Services, Medical Services and Techniques Department, First and Emergency Aid Programme, Bayburt University, Bayburt, 69000, Turkey.
Aim: Identifying the risks of metabolic syndrome (MetS) can lead to early targeted interventions and thus contribute to improved quality of life by reducing the risk of developing MetS, diabetes or heart disease in the future. We aimed to develop a valid and reliable measurement tool to measure the MetS risk of the population.
Materials And Methods: In the methodological study, an item pool was created by reviewing the literature.
High-resolution melting analysis for detection of nucleotide mutation markers in the polymerase-acidic (PA) gene of influenza virus that are associated with baloxavir marboxil resistance.
Arch Virol
January 2025
Department Experimental and Clinical Medicine, University of Florence, Florence, Italy.
The I38T substitution in the influenza virus polymerase-acidic (PA) subunit is a resistance marker of concern for treatment with the antiviral baloxavir marboxil (BXM). Thus, monitoring PA/I38T mutations is of clinical importance. Here, we developed three rapid and sensitive assays for the detection and monitoring of the PA/I38T mutation.
View Article and Find Full Text PDFDevelopment of a Prediction Model and Risk Score for Self-Assessment and High-Risk Population Identification in Liver Cancer Screening: Prospective Cohort Study.
JMIR Public Health Surveill
December 2024
Department of Cancer Prevention, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine, Chinese Academy of Sciences, 1 East Banshan Road, Hangzhou, 310022, China, 86 571-88122219.
Background: Liver cancer continues to pose a significant burden in China. To enhance the efficiency of screening, it is crucial to implement population stratification for liver cancer surveillance.
Objective: This study aimed to develop a simple prediction model and risk score for liver cancer screening in the general population, with the goal of improving early detection and survival.
Classification of ROI-based fMRI data in short-term memory tasks using discriminant analysis and neural networks.
Front Neuroinform
December 2024
Institute of Theoretical Physics, Jagiellonian University, Kraków, Poland.
Understanding brain function relies on identifying spatiotemporal patterns in brain activity. In recent years, machine learning methods have been widely used to detect connections between regions of interest (ROIs) involved in cognitive functions, as measured by the fMRI technique. However, it's essential to match the type of learning method to the problem type, and extracting the information about the most important ROI connections might be challenging.
View Article and Find Full Text PDFEffect of Nutrition in the First Two Years of Life on Body Composition of Preschool-Aged Children.
Cureus
December 2024
Pediatrics, Assiut University, Assiut, EGY.
Background Feeding and growth during infancy have been associated with later life body mass index and early excessive weight gain is associated with obesity later on. This study aimed to assess the effect of feeding in the first two years of life on the body composition of children at the preschool age and detect the importance of using bioelectrical impedance (BIA) analysis in identifying individuals at risk of overweight and obesity. Methods A cross-sectional study included 160 children.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!