AI Article Synopsis
- The study focused on analyzing mitochondrial DNA sequence variability in four specific regions across different species of the Contracaecum nematodes from various countries.* -
- The researchers used PCR amplification and sequencing techniques to measure genetic differences, finding low variability within species but significantly higher variation between different species.* -
- Phylogenetic analysis indicated that specimens of the same Contracaecum species grouped together, supporting the idea of sibling species within the C. rudolphii group.*
Article Abstract
The present study investigated sequence variability in four mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit (cox1), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), and small subunit of rRNA (rrnS), among Contracaecum rudolphii A, C. rudolphii B, C. rudolphii C and Contracaecum septentrionale from different hosts and geographical origins in China, Italy, Spain and the USA. Regions in the cox1, nad1, nad4 and rrnS genes (designated pcox1, pnad1, pnad4 and prrnS, respectively) were amplified separately from individual nematodes by PCR, sequenced and compared to estimate sequence variability. While sequence variation within each of the Contracaecum species was 0-2.6% for pcox1, 0.3-2.5% for pnad1, 0-1.9% for pnad4 and 0-2.9% for prrnS, differences between species was significantly higher, being 3.3-12%, 9.8-15.2%, 9.6-18.3% and 3.5-11.12% for these regions, respectively. Phylogenetic analyses of pcox1, pnad1, pnad4 and prrnS sequence data using maximum likelihood (ML), maximum parsimony (MP) and neighbour joining (NJ) showed that the specimens of each Contracaecum species clustered together. These results provide additional genetic evidence for the existence of sibling species within C. rudolphii sensu lato.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mcp.2013.02.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Online monitoring and stable isotope tracing of cancer associated volatiles in murine model captures tumor associated markers in vivo.
Anal Chim Acta
May 2025
Department of Human Sciences, The Ohio State University, USA; James Comprehensive Cancer Center, The Ohio State University, Columbus, OH, 43210, USA. Electronic address:
Background: The imperative need for early cancer detection, which is crucial for improved survival rates in many severe cancers such as lung cancer, remains challenging due to the lack of reliable early-diagnosis technologies and robust biomarkers. To address this gap, innovative screening platforms are essential to unveil the chemical signatures of lung cancer and its treatments. It is established that the oxidative tumor environment induces alterations in host metabolic processes and influences endogenous volatile synthesis.
View Article and Find Full Text PDFGenetic diversity and population structure analysis of Indian blackberry (Syzygium cumini L.) using CAAT box‑derived polymorphism (CBDP) and start codon targeted polymorphism (SCoT) markers.
J Genet Eng Biotechnol
March 2025
ICAR- Central Institute for Subtropical Horticulture, Lucknow 226101, India.
Indian blackberry (Syzygium cumini L.) also known as jamun is a very important underutilized fruit crop with notable medicinal and economic value. However, its genetic improvement has been constrained by limited knowledge of the genetic diversity within existing collections.
View Article and Find Full Text PDFMorphological and genetic diversity assessment of cultivated Thymus satureioides (endemic species) of Morocco.
J Genet Eng Biotechnol
March 2025
National Institute of Agricultural Research (INRA), Regional Center of Agricultural Research Rabat, Biotechnology Unit, Rabat 10000 Morocco.
Thymus satureioides is an endemic and medicinal plant of Morocco, widely distributed in the arid and semiarid habitats. Communally used in traditional medicine. In the current study, twelve Inter-Simple Sequence Repeats (ISSR) primers combined with 11 agro-morphological traits were applied to evaluate 60 accessions of T.
View Article and Find Full Text PDFLarge-scale genetic mapping for human brain asymmetry.
Handb Clin Neurol
March 2025
Language & Genetics Department, Max Planck Institute for Psycholinguistics, Nijmegen, The Netherlands; Department of Cognitive Neuroscience & Donders Community for Medical Neuroscience, Radboud University Medical Center, Nijmegen, The Netherlands. Electronic address:
Left-right asymmetry is an important aspect of human brain organization for functions including language and hand motor control, which can be altered in some psychiatric traits. The last 5 years have seen rapid advances in the identification of specific genes linked to variation in asymmetry of the human brain and/or handedness. These advances have been driven by a new generation of large-scale genome-wide association studies, carried out in samples ranging from roughly 16,000 to over 1.
View Article and Find Full Text PDFThe Role of Mitochondrial DNA Copy Number in Venous Thromboembolism: Insights from a 2-Sample Mendelian Randomization Study.
Ann Vasc Surg
April 2025
Department of Cardiology, Dongying People's Hospital (Dongying Hospital of Shandong Provincial Hospital Group), Dongying, China. Electronic address:
Background: Venous thromboembolism (VTE), including pulmonary embolism (PE) and deep vein thrombosis (DVT), is the third most common cardiovascular disease. A low amount of mitochondrial DNA copy number (mtDNA-CN) reflects mitochondrial dysfunctions and has been associations with arterial cardiovascular diseases. However, the role of mtDNA-CN in venous cardiovascular disease was unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!