Background: Pearl millet [Pennisetum glaucum (L.) R. Br.] is a widely cultivated drought- and high-temperature tolerant C4 cereal grown under dryland, rainfed and irrigated conditions in drought-prone regions of the tropics and sub-tropics of Africa, South Asia and the Americas. It is considered an orphan crop with relatively few genomic and genetic resources. This study was undertaken to increase the EST-based microsatellite marker and genetic resources for this crop to facilitate marker-assisted breeding.
Results: Newly developed EST-SSR markers (99), along with previously mapped EST-SSR (17), genomic SSR (53) and STS (2) markers, were used to construct linkage maps of four F7 recombinant inbred populations (RIP) based on crosses ICMB 841-P3 × 863B-P2 (RIP A), H 77/833-2 × PRLT 2/89-33 (RIP B), 81B-P6 × ICMP 451-P8 (RIP C) and PT 732B-P2 × P1449-2-P1 (RIP D). Mapped loci numbers were greatest for RIP A (104), followed by RIP B (78), RIP C (64) and RIP D (59). Total map lengths (Haldane) were 615 cM, 690 cM, 428 cM and 276 cM, respectively. A total of 176 loci detected by 171 primer pairs were mapped among the four crosses. A consensus map of 174 loci (899 cM) detected by 169 primer pairs was constructed using MergeMap to integrate the individual linkage maps. Locus order in the consensus map was well conserved for nearly all linkage groups. Eighty-nine EST-SSR marker loci from this consensus map had significant BLAST hits (top hits with e-value ≤ 1E-10) on the genome sequences of rice, foxtail millet, sorghum, maize and Brachypodium with 35, 88, 58, 48 and 38 loci, respectively.
Conclusion: The consensus map developed in the present study contains the largest set of mapped SSRs reported to date for pearl millet, and represents a major consolidation of existing pearl millet genetic mapping information. This study increased numbers of mapped pearl millet SSR markers by >50%, filling important gaps in previously published SSR-based linkage maps for this species and will greatly facilitate SSR-based QTL mapping and applied marker-assisted selection programs.
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http://dx.doi.org/10.1186/1471-2164-14-159 | DOI Listing |
Plants (Basel)
December 2024
School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Australia.
This study assessed the effectiveness of four competitive pasture species-Premier digit grass ( Steud. var. Premier), Rhodes grass ( Kunth.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Laboratoire de recherche sur les produits bioactifs et valorisation de la biomasse, Ecole Normale Supérieure de Kouba cheikh Mohamed elbachir ElIbrahimi, B.P. 92, 16308 Vieux-Kouba, Algiers, Algeria. Electronic address:
This study aimed to use a new protein complex of Pennisetin (Pen) a non gluten protein of pearl millet and casein (Cas), for curcumin (Cur) extract encapsulation using simple or complex coacervation. The potential improvement of Cur antioxidant activities and α-amylase inhibition after encapsulation was explored. Complex microparticles of Pen and Cas with various ratios exhibited average diameters ranging from 1.
View Article and Find Full Text PDFArch Microbiol
January 2025
Agricultural Botany Department, Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt.
Researchers have reported that Bacillus megaterium BM18-2 reduces Cd toxicity in Hybrid Pennisetum, but understanding the interaction between plants and associated endophytes is crucial for understanding phytoremediation strategies under heavy metal stress. The current study aims to monitor the colonization patterns of GFP-labeled endophytic bacteria BM18-2 on Hybrid Pennisetum grass. Additionally, it will monitor Cd's effect on plant bacterial colonization.
View Article and Find Full Text PDFIntroduction: The increase in vapor pressure deficit (VPD) is among the expected change in futur climate, and understanding its effect on crop growth is of much significance for breeeding programs. Three groups (G1,G2 and G3) of pearl millet germplasm, originating from regions with different rainfall intensities, were grown in the field during period of high and low VPDs. The groups G1,G2 and G3 were respectively from Guinean (rainfall above 1000 mm), Soudanian (rainfall between 600 mm and 900 mm), and Sahelian zones (rainfall between 600 and 300 mm) of Africa.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
London Research and Development Centre, Agriculture and Agri-Food Canada, Vineland Station, ON L0R 2E0, Canada.
Agricultural soil environments contain different types of nematodes in all trophic levels that aid in balancing the soil food web. Beneficial free-living nematodes (FLNs) consist of bacterivores, fungivores, predators, and omnivores that help in the mineralization of the soil and the top-down control of harmful plant-parasitic nematodes (PPNs). Annually, USD 125 billion in worldwide crop losses are caused by PPNs, making them a plant pathogen of great concern for growers.
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