Objective: To provide reliable seed cells with unlimited passage proliferation and stable biological characteristics for periodontal tissue engineering research through infecting a retrovirus carrying SV40Tag into SD rat dental follicle cells.
Methods: Retroviral virus vector containing SV40Tag by 293 cells was packaged and infected into SD rat dental follicle cells. Normal dental capsule cells were used as control group. The cell morphology and vitality were observed by inverted microscope, telomerase activity, osteogenic differentiation and proliferation of dental follicle cells were analyzed.
Results: The SD rat dental follicle cells infected with SV40Tag could be passaged for 60 generations in vitro culture with strong growth activity. The telomerase activity was significantly enhanced compared with the control group (P = 0.033). The expression of alkaline phosphatase, osteocalcin, bone morphogenesis protein-2, Runx2, basic fibroblast growth factor, insulin-like growth factors had no statistical difference compared with control cells (P > 0.05).
Conclusion: The SD rat dental follicle cells infected with SV40Tag not only have a strong growth activity and infinite passaged capacity, but also have a stable biological property as normal dental follicle cells, so it can be regarded as the excellent seed cells in periodontal tissue engineering.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Significant response to tocilizumab in a case of immune deposits-related membranoproliferative glomerulonephritis and tubulointerstitial nephritis complicated by multicentric Castleman's disease.
Clin Nephrol Case Stud
December 2024
Nephrology Center and the Okinaka Memorial Institute for Medical Research.
A 47-year-old woman with a 12-year history of anemia and high C-reactive protein (CRP) levels was admitted to our hospital with worsening fatigue and night sweats. She had high levels of immunoglobulin G (IgG; 4182 mg/dL), IgA (630.6 mg/dL), and CRP (7.
View Article and Find Full Text PDFCaries Level in 3-Year-Olds in Germany: National Caries Trends and Gaps in Primary Dental Care.
Children (Basel)
November 2024
Pediatric Dentistry Department, University Medicine of Greifswald, 17475 Greifswald, Germany.
Background: Nationally representative long-term data on caries in the primary dentition are rare but essential for determining the need for prevention and treatment. This research assessed the prevalence and trends of dental caries in 3-year-old children across Germany, with national data analyzed and compared with the corresponding data for 6-7-year-olds.
Methods: Data were extracted from the most recent German National Oral Health Survey in 2016.
Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst.
Diagn Pathol
January 2025
Cell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.
Background: Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst.
Methods: Immunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs).
Results: The immunohistochemical expression of these proteins was predominantly cytoplasmic and granular across all samples.
Gallic acid-guar gum and chitosan-based polyelectrolyte complex film exhibited enhanced wound healing in full-thickness excision wound model.
J Biomater Sci Polym Ed
January 2025
Department of Medical Affairs, Curie Sciences, Samastipur, Bihar, India.
Article Synopsis
- Recent developments in wound dressings focus on natural bioactives like guar gum (GG) and gallic acid (GA), which can enhance healing compared to traditional materials.
- The study created a GA-GG conjugate through a polymerization process, confirming its successful integration and beneficial structural changes via various spectroscopic analyses.
- A polyelectrolyte complex (PEC) film made with chitosan showed strong antioxidant and antimicrobial properties, high swelling rates, and excellent wound healing efficacy, achieving 94.5% closure in comparison to untreated controls.
Deciphering odontogenic myxoma: the role of copy number variations as diagnostic signatures.
J Zhejiang Univ Sci B
December 2024
Department of Oral Pathology, Peking University School and Hospital of Stomatology / National Center of Stomatology / National Clinical Research Center for Oral Diseases / National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China.
In light of the lack of reliable molecular markers for odontogenic myxoma (OM), the detection of copy number variation (CNV) may present a more objective method for assessing ambiguous cases. In this study, we employed multiregional microdissection sequencing to integrate morphological features with genomic profiling. This allowed us to reveal the CNV profiles of OM and compare them with dental papilla (DP), dental follicle (DF), and odontogenic fibroma (OF) tissues.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!