Resazurin-based assay for screening bacteria for radiation sensitivity.

Springerplus

Department of Microbiology and Immunology, AT Still University of Health Sciences, Kirksville, MO USA.

Published: December 2013

We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586403PMC
http://dx.doi.org/10.1186/2193-1801-2-55DOI Listing

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