Aim: This study aimed to evaluate the influence of phenothiazine derivatives (PDs) on the intracellular accumulation of cyanine dye DiOC6(3) in doxorubicin-resistant LoVo-DX cell line, with overexpression of P-glycoprotein.
Materials And Methods: In order to maintain a high expression level of P-gp, the LoVo-DX cells were grown in the presence of doxorubicin (100 ng/ml). The time-dependent fluorescence signal (T-DFS) of the intracellular accumulation of DiOC6(3), in the presence of PDs, was then recorded. The rate constants k1, k2, k3 and amplitudes of T-DFS, describing the intracellular accumulation process, were determined based on the respective theoretical equation.
Results: The values of k1 and k2 were dependent on the hydrophobicity (logP) of the PDs used as drug resistance modulators. A rise of k1 and k2 values was observed when the logP of PDs increased.
Conclusion: We suggest that the k1 and k2 rate constants could be regarded as useful parameters for assessment of PDs as well as of other compounds of potential application as reversers of multidrug resistance.
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